Linked Life Data

9585817

Source:http://linkedlifedata.com/resource/pubmed/id/9585817

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-6-3
pubmed:abstractText
Antibodies against the HuD antigen expressed in small-cell lung cancer (SCLC) cross-react with proteins expressed in neurons of the central and peripheral nervous system and are associated with paraneoplastic encephalomyelitis and sensory neuropathy (PEM/SN). We isolated anti-HuD Fab fragments from an antibody phage display library that was constructed from mRNA of a metastatic lymph node from a patient with SCLC and Pem/SN. Fab GLN495 recognized HuD and other related proteins (HuC and Hel-N1, or Hu antigens) in immunoblots of these recombinant proteins and in immunohistochemical and Western blot analysis of SCLC and neurons. Fab GLN495 inhibited up to 75% of the anti-Hu antibodies of the patient from which it was derived, suggesting that recognizes a dominant epitope in the polyclonal anti-Hu antibody response. Fab GLN495 also competed with anti-Hu sera from most but not all patients with PEM/SN, indicating that the same epitope is recognized by a large subgroup of patients. Human monoclonal anti-HuD antibodies may be useful in diagnosis of HuD expressing tumors and in clarifying the autoimmune etiology of PEM/SN. This study, the first to demonstrate that tumor specific recombinant antibodies can be isolated from metastatic lymph node tissue, shows that this approach may be generally applicable to isolate human antibodies against tumor specific antigens.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-5728
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
200-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9585817-Amino Acid Sequence, pubmed-meshheading:9585817-Antibodies, pubmed-meshheading:9585817-Bacteriophages, pubmed-meshheading:9585817-Carcinoma, Small Cell, pubmed-meshheading:9585817-Encephalomyelitis, pubmed-meshheading:9585817-Epitopes, pubmed-meshheading:9585817-Gene Deletion, pubmed-meshheading:9585817-Hu Paraneoplastic Encephalomyelitis Antigens, pubmed-meshheading:9585817-Humans, pubmed-meshheading:9585817-Immunoglobulin Fab Fragments, pubmed-meshheading:9585817-Lung Neoplasms, pubmed-meshheading:9585817-Molecular Sequence Data, pubmed-meshheading:9585817-Nerve Tissue Proteins, pubmed-meshheading:9585817-Neurons, pubmed-meshheading:9585817-Paraneoplastic Syndromes, pubmed-meshheading:9585817-RNA-Binding Proteins, pubmed-meshheading:9585817-Recombinant Proteins
pubmed:year
1998
pubmed:articleTitle
Selection of recombinant anti-HuD Fab fragments from a phage display antibody library of a lung cancer patient with paraneoplastic encephalomyelitis.
pubmed:affiliation
Department of Immunology, Scripps Research Institute, La Jolla, CA USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't