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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-6-2
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pubmed:databankReference |
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pubmed:abstractText |
The synaptic protein PSD-95/SAP90 binds to and clusters a variety of membrane proteins via its two N-terminal PDZ domains. We report a novel protein, CRIPT, which is highly conserved from mammals to plants and binds selectively to the third PDZ domain (PDZ3) of PSD-95 via its C terminus. While conforming to the consensus PDZ-binding C-terminal sequence (X-S/T-X-V-COOH), residues at the -1 position and upstream of the last four amino acids of CRIPT determine its specificity for PDZ3. In heterologous cells, CRIPT causes a redistribution of PSD-95 to microtubules. In brain, CRIPT colocalizes with PSD-95 in the postsynaptic density and can be coimmunoprecipitated with PSD-95 and tubulin. These findings suggest that CRIPT may regulate PSD-95 interaction with a tubulin-based cytoskeleton in excitatory synapses.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DLG2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DLG4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SAPAP proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/postsynaptic density proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0896-6273
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
693-707
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9581762-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:9581762-Amino Acid Sequence,
pubmed-meshheading:9581762-Animals,
pubmed-meshheading:9581762-Arabidopsis,
pubmed-meshheading:9581762-Arabidopsis Proteins,
pubmed-meshheading:9581762-Binding Sites,
pubmed-meshheading:9581762-Brain,
pubmed-meshheading:9581762-Caenorhabditis,
pubmed-meshheading:9581762-Caenorhabditis elegans Proteins,
pubmed-meshheading:9581762-Carrier Proteins,
pubmed-meshheading:9581762-Cells, Cultured,
pubmed-meshheading:9581762-Cloning, Molecular,
pubmed-meshheading:9581762-Consensus Sequence,
pubmed-meshheading:9581762-Guanylate Kinase,
pubmed-meshheading:9581762-Hippocampus,
pubmed-meshheading:9581762-Humans,
pubmed-meshheading:9581762-Immunohistochemistry,
pubmed-meshheading:9581762-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:9581762-Male,
pubmed-meshheading:9581762-Membrane Proteins,
pubmed-meshheading:9581762-Molecular Sequence Data,
pubmed-meshheading:9581762-Nerve Tissue Proteins,
pubmed-meshheading:9581762-Neurons,
pubmed-meshheading:9581762-Rats,
pubmed-meshheading:9581762-Rats, Sprague-Dawley,
pubmed-meshheading:9581762-Recombinant Proteins,
pubmed-meshheading:9581762-Sequence Alignment,
pubmed-meshheading:9581762-Sequence Homology, Amino Acid,
pubmed-meshheading:9581762-Synapses,
pubmed-meshheading:9581762-Synaptosomes,
pubmed-meshheading:9581762-Tumor Suppressor Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
CRIPT, a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90.
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pubmed:affiliation |
Department of Neurobiology and Howard Huges Medical Institute, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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