Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-6-9
pubmed:abstractText
A correlation was found between the expression of a specific Mycoplasma fermentans surface antigen (Pra, proteinase-resistant antigen) and the site of isolation of the organism from the infected host. Strains which expressed Pra were most frequently associated with cells of bone marrow origin, and strains which lacked expression of Pra were most commonly isolated from the respiratory tract, genital tract, and arthritic joints, i.e., epithelial cell surfaces. Pra was previously shown to be resistant to degradation by proteinases and was hypothesized to play a protective role at the organism surface and perhaps to influence which host tissue site was colonized by the organism. The methods used for this phenotyping scheme required isolation and growth of the mycoplasma in quantities sufficient for immunoblot analysis using monoclonal antibodies. We wanted to determine a more rapid and less cumbersome technique to supplement this method for determining the Pra phenotype directly in clinical specimens. Here we describe PCR studies to investigate the movement of a previously identified M. fermentans insertion sequence (IS)-like element. These data showed a correlation between a specific IS genotype and the Pra+ phenotype. Production of a 160-bp product using a single set of IS-based primers was associated with expression of Pra. The genomic IS location resulting in the 160-bp product was determined by using Southern blot analysis and was found to be a stable insertion site characteristic of genotype I strains. Additional analyses of sequences within and flanking the IS insertion sites revealed another pair of PCR primer sites which resulted in the consistent production of a 450-bp amplicon. The stability of this site was dependent on the absence of the IS-like element between the primer sites. The production of this 450-bp amplicon correlated with the Pra mutant phenotype and was characteristic of genotype II strains. The data showed that the sequence within the IS may be unstable and that reliable genotyping sequences are more easily found in the stable genomic sites which flank the IS element.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-1310331, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-1371767, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-1705362, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-1812547, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-1975457, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2172089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2323816, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2390096, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2432017, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2437033, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2449398, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2497174, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2676320, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2712198, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2786191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2802022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2817215, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-2817216, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-309436, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3097812, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3122651, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3281909, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3312097, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3486159, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-3728790, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-4203529, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-4427082, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-4929377, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-4964009, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-5007551, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-5219024, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-5833637, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-8606148, http://linkedlifedata.com/resource/pubmed/commentcorrection/9574708-8626289
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1371-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Genotypic and phenotypic analysis of Mycoplasma fermentans strains isolated from different host tissues.
pubmed:affiliation
Department of Microbiology, University of Alabama at Birmingham, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.