Linked Life Data

9572274

Source:http://linkedlifedata.com/resource/pubmed/id/9572274

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-5-18
pubmed:abstractText
Findings in vivo and in culture suggest that neuronal activity selectively regulates GABA(A) receptor delta subunit mRNA expression in cerebellar granule neurons. For example, the onset of delta subunit mRNA expression during postnatal maturation coincides with innervation. Furthermore, depolarizing conditions (25 mM KCl) in culture initiate and maintain increases in the delta subunit transcript level. We have now examined whether similar changes in delta subunit mRNA expression occur in cultured neurons after activation of glutamate receptors of the NMDA subtype, an event that mimics granule neuron depolarization by mossy fiber innervation in vivo. Our studies demonstrate that addition of 50 microM NMDA to cultured rat granule neurons maintained in defined, serum-free medium specifically initiates delta subunit transcript expression. Whereas the level of the delta subunit mRNA is increased fourfold by this treatment, levels of other GABA(A) receptor subunit transcripts are not significantly changed. The level of the delta subunit transcript is further increased when NMDA receptor activation is enhanced by maintaining neurons in a Mg2+-free medium to alleviate Mg2+ blockade of the receptor channel. The NMDA-induced elevation in delta subunit transcript expression involves activation of a Ca2+/calmodulin-dependent protein kinase pathway. These findings suggest that activation of an excitatory pathway may regulate the expression of an inhibitory receptor phenotype in cerebellar granule neurons in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1907-15
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
NMDA receptor stimulation selectively initiates GABA(A) receptor delta subunit mRNA expression in cultured rat cerebellar granule neurons.
pubmed:affiliation
Department of Neurosciences, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106-4965, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.