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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-5-7
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pubmed:abstractText |
To determine whether human CD4+ T cells undergo post-thymic maturation, we compared the susceptibility to anergy induction in human thymic CD1a- CD4+ single-positive (CD4+), cord blood (CB) CD4+, and adult peripheral blood (APB) CD4+ T cells by stimulation with toxic shock syndrome toxin-1 (TSST-1). Most TSST-1-induced T cell blasts derived from either T cell preparation expressed TCR Vbeta2, which determines the potential reactivity to TSST-1. Most thymic CD4+ T cell blast preparations exhibited little or no production of IL-2 and IL-4 after restimulation with TSST-1 and only marginal responses after stimulation with rIL-2 or a combination of PMA and calcium ionophore, while the APB CD4+ T cell blasts showed high responses to these stimuli. The responses of CB CD4+ T cell blasts to these stimuli varied, ranging from minimal to relatively high. Studies of DNA fragmentation showed that there was no significant cell death of thymic CD4+ T cell blasts. Most thymic CD1a- CD4+ and CB CD4+ T cells were CD38 positive. APB CD4+ T cell blasts derived from the CD38+ fraction and from the CD38- fraction exhibited equally high responses to restimulation with TSST-1. These results indicate that thymic CD1a- CD4+ and CB CD4+ T cells are inherently highly susceptible to anergy induction by bacterial superantigens and that thymic CD1a- CD4+ T cells are less mature than CB CD4+ T cells, suggesting that post-thymic maturation in thymic T cells migrating to the periphery is required for acquisition of full reactivity to antigenic stimulation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/NAD Nucleosidase,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin F, Staphylococcal
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
160
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
112-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9551963-ADP-ribosyl Cyclase,
pubmed-meshheading:9551963-Adolescent,
pubmed-meshheading:9551963-Adult,
pubmed-meshheading:9551963-Antigens, CD,
pubmed-meshheading:9551963-Antigens, CD1,
pubmed-meshheading:9551963-Antigens, CD38,
pubmed-meshheading:9551963-Antigens, Differentiation,
pubmed-meshheading:9551963-Bacterial Toxins,
pubmed-meshheading:9551963-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9551963-Cell Survival,
pubmed-meshheading:9551963-Child,
pubmed-meshheading:9551963-Child, Preschool,
pubmed-meshheading:9551963-Clonal Anergy,
pubmed-meshheading:9551963-Enterotoxins,
pubmed-meshheading:9551963-Fetal Blood,
pubmed-meshheading:9551963-Humans,
pubmed-meshheading:9551963-Immunophenotyping,
pubmed-meshheading:9551963-Infant,
pubmed-meshheading:9551963-Interleukin-2,
pubmed-meshheading:9551963-Interleukin-4,
pubmed-meshheading:9551963-Lymphocyte Activation,
pubmed-meshheading:9551963-Membrane Glycoproteins,
pubmed-meshheading:9551963-NAD+ Nucleosidase,
pubmed-meshheading:9551963-Superantigens,
pubmed-meshheading:9551963-Th2 Cells,
pubmed-meshheading:9551963-Thymus Gland
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pubmed:year |
1998
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pubmed:articleTitle |
Post-thymic maturation of migrating human thymic single-positive T cells: thymic CD1a- CD4+ T cells are more susceptible to anergy induction by toxic shock syndrome toxin-1 than cord blood CD4+ T cells.
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pubmed:affiliation |
Department of Microbiology and Immunology, The Heart Institute of Japan, Tokyo Women's Medical College, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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