Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1998-4-21
|
| pubmed:abstractText |
The CC chemokine eotaxin, originally purified from bronchoalveolar lavage fluid of sensitized guinea pigs following allergen challenge, is a potent eosinophil-selective chemoattractant. In the present study, we have used (111)In-eosinophils and human eotaxin to characterize the profile of chemokine-induced eosinophil accumulation in vivo in rat skin. Intradermally injected eotaxin caused a dose-dependent accumulation of (111)In-eosinophils. Time course studies indicated that the response was rapid, since all the accumulation occurred within the first 1 to 2 h of eotaxin injection. The i.v. administration of anti-intercellular adhesion molecule-1, anti-vascular cell adhesion molecule-1, or anti-alpha4 integrin mAbs significantly inhibited the eosinophil accumulation induced by 100 pmol of human eotaxin by 73, 43, and 67%, respectively. Further, when (111)In-eosinophils were pretreated in vitro with anti-alpha4 integrin or anti-beta2 integrin mAbs, or with a combination of both mAbs, eotaxin-induced responses in vivo were reduced by 52, 49, and 68%, respectively. Eosinophil accumulation induced by intradermal IL-4, but not that induced by TNF-alpha or leukotriene B4, appeared to be mediated in part by endogenously generated eotaxin. Anti-eotaxin Abs significantly inhibited (54%) the later phases (24-28 h) but not the early phase (0-4 h) of the response to IL-4. This was consistent with eotaxin mRNA expression peaking at 18 h after IL-4 injection. Our findings show that human eotaxin is a potent inducer of eosinophil accumulation in vivo, this response being dependent on alpha4 integrin/vascular cell adhesion molecule-1 and beta2 integrin/intercellular adhesion molecule-1 adhesion pathways. Further, the eosinophil accumulation in response to IL-4 is partly mediated by endogenously generated eotaxin.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/CCL11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Ccl11 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL11,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors, Eosinophil,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Indium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4,
http://linkedlifedata.com/resource/pubmed/chemical/Integrins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
160
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3569-76
|
| pubmed:dateRevised |
2010-8-25
|
| pubmed:meshHeading |
pubmed-meshheading:9531320-Animals,
pubmed-meshheading:9531320-Antibodies, Monoclonal,
pubmed-meshheading:9531320-Antigens, CD,
pubmed-meshheading:9531320-Antigens, CD18,
pubmed-meshheading:9531320-Calcium,
pubmed-meshheading:9531320-Cell Movement,
pubmed-meshheading:9531320-Chemokine CCL11,
pubmed-meshheading:9531320-Chemokines, CC,
pubmed-meshheading:9531320-Chemotactic Factors, Eosinophil,
pubmed-meshheading:9531320-Cytokines,
pubmed-meshheading:9531320-Edema,
pubmed-meshheading:9531320-Eosinophils,
pubmed-meshheading:9531320-Humans,
pubmed-meshheading:9531320-Indium Radioisotopes,
pubmed-meshheading:9531320-Injections, Intradermal,
pubmed-meshheading:9531320-Injections, Intravenous,
pubmed-meshheading:9531320-Integrin alpha4,
pubmed-meshheading:9531320-Integrins,
pubmed-meshheading:9531320-Interleukin-4,
pubmed-meshheading:9531320-Intracellular Fluid,
pubmed-meshheading:9531320-Male,
pubmed-meshheading:9531320-Rats,
pubmed-meshheading:9531320-Rats, Sprague-Dawley,
pubmed-meshheading:9531320-Skin,
pubmed-meshheading:9531320-Time Factors
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Human eotaxin induces alpha 4 and beta 2 integrin-dependent eosinophil accumulation in rat skin in vivo: delayed generation of eotaxin in response to IL-4.
|
| pubmed:affiliation |
Leukocyte Biology Centre, Biomedical Sciences Division, Imperial College School of Medicine, London, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|