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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1998-5-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
A novel antigen was identified by the cross-reactivity of the anti-CD30 antibody Ki-1. This 57-kD intracellular Ki-1 antigen (Ki-1/57) is induced upon activation of leukocytes and is transported to the nuclear compartment. We describe the partial cloning and sequencing of the Ki-1/57 cDNA from a lambda gt 11-cDNA library derived from the Hodgkin-analogous cell line L540. New monoclonal antibodies were produced against the recombinant Ki-1/57 protein fragment which were used to confirm that the Ki-1/57 antigen is associated with kinase activity and is expressed in a variety of tumor cell lines and in activated but not resting leukocytes. The Ki-1/57 gene was mapped to the bands 9q22.3-31 of human chromosome 9. This is an area which appears to be associated with secondary chromosomal aberrations in acute myeloid leukemias.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0254-9670
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
273-80
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9523163-Amino Acid Sequence,
pubmed-meshheading:9523163-Animals,
pubmed-meshheading:9523163-Antigens, CD30,
pubmed-meshheading:9523163-Baculoviridae,
pubmed-meshheading:9523163-Base Sequence,
pubmed-meshheading:9523163-Cell Line,
pubmed-meshheading:9523163-Chromosome Banding,
pubmed-meshheading:9523163-Chromosomes, Human, Pair 9,
pubmed-meshheading:9523163-Cloning, Molecular,
pubmed-meshheading:9523163-DNA, Complementary,
pubmed-meshheading:9523163-Humans,
pubmed-meshheading:9523163-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9523163-Mice,
pubmed-meshheading:9523163-Mice, Inbred BALB C,
pubmed-meshheading:9523163-Molecular Sequence Data,
pubmed-meshheading:9523163-Molecular Weight,
pubmed-meshheading:9523163-Restriction Mapping,
pubmed-meshheading:9523163-Spodoptera
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Characterization, mapping and partial cDNA sequence of the 57-kD intracellular Ki-1 antigen.
|
| pubmed:affiliation |
Bristol-Myers Squibb, Pharmaceutical Research Institute, Princeton, N.J. 08543-4000, USA. Jorg_Kobarg@ccmail.bms.com
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|