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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1998-5-1
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pubmed:abstractText |
We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3'-deoxynucleoside triphosphate (3'-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in <30 min, to reduce the amount of template required, and to do PCR direct sequencing without the elimination of primers and 2'-dNTP, which disturbs the Sanger sequencing reaction. An accurate and longer read length was made possible by newly designed four-color dye-3'-dNTPs and mutated RNA polymerase with an improved incorporation rate of 3'-dNTP. This method should be useful for large-scale sequencing in genome projects and clinical diagnosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-1371869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-1416049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-1598205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-1655254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-1702976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2030975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2159476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2204707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2443975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-2744488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-3002422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-3200828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-3474623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-3839756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-3951988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7479895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7603992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7688864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7753809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7915835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-7937169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-8618879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9520387-9074923
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:CarninciPP,
pubmed-author:HayashizakiYY,
pubmed-author:IzawaMM,
pubmed-author:MatsuuraSS,
pubmed-author:MuramatsuMM,
pubmed-author:OkazakiYY,
pubmed-author:OzawaKK,
pubmed-author:SasakiNN,
pubmed-author:TanakaTT,
pubmed-author:WatahikiMM,
pubmed-author:YonedaYY
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3455-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1998
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pubmed:articleTitle |
Transcriptional sequencing: A method for DNA sequencing using RNA polymerase.
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pubmed:affiliation |
Genome Science Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research, 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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