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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-5-26
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pubmed:abstractText |
A Pichia pastoris expression clone has been developed to produce the human cytokine interleukin-17 (hIL-17). Characterization of purified recombinant hIL-17 made with this clone demonstrated that it shared many characteristics with hIL-17 produced in mammalian cells. The hIL-17 produced in Pichia had the correct N-terminus of natural mature hIL-17 and a glycosylation pattern similar to hIL-17 produced in mammalian cells; both Pichia and human cells add approximately 5 kDa of sugars via N-linked glycosylation and both express a mixture of the glycosylated and nonglycosylated forms. Gel filtration provides evidence that the Pichia produced hIL-17 exists as a dimer in solution. A combination of cation-exchange and gel-filtration chromatography yielded 3.5 mg of highly purified and biologically active hIL-17 from a 10-liter fermentation. These results show that P. pastoris is a useful system to produce recombinant hIL-17 in structure/function studies of this molecule.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1046-5928
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
208-14
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9518462-Amidohydrolases,
pubmed-meshheading:9518462-Base Sequence,
pubmed-meshheading:9518462-Cytokines,
pubmed-meshheading:9518462-DNA Primers,
pubmed-meshheading:9518462-Dimerization,
pubmed-meshheading:9518462-Dose-Response Relationship, Drug,
pubmed-meshheading:9518462-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9518462-Fibroblasts,
pubmed-meshheading:9518462-Glycoproteins,
pubmed-meshheading:9518462-Humans,
pubmed-meshheading:9518462-Interleukin-17,
pubmed-meshheading:9518462-Interleukin-6,
pubmed-meshheading:9518462-Interleukins,
pubmed-meshheading:9518462-Molecular Sequence Data,
pubmed-meshheading:9518462-Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase,
pubmed-meshheading:9518462-Pichia,
pubmed-meshheading:9518462-Polymerase Chain Reaction,
pubmed-meshheading:9518462-Recombinant Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Expression of human interleukin-17 in Pichia pastoris: purification and characterization.
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pubmed:affiliation |
Endogen, Inc., Woburn, Massachusetts 01801-1059, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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