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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-4-8
|
| pubmed:abstractText |
Cardiac myxoma cells produce large amounts of interleukin (IL)-6 and IL-8. To determine whether immunosuppressive agents could be used to treat cardiac myxoma, we tested the effects of dexamethasone and three of the newer second-generation immunosuppressive drugs, cyclosporin A, tacrolimus, and deoxyspergualin, on the production of IL-6 and IL-8 in these cells. Cultured cardiac myxoma cells were used as in vitro model of cardiac myxoma. Cells were tested for 24 hours with 10(-7) M dexamethasone, 10(-6) M cyclosporin A, 10(-8) M tacrolimus, and 10(-6) M 15-deoxyspergualin, with aliquots of conditioned medium being assayed for cytokine levels at 0, 6, 12, and 24 hours. Cardiac myxoma cells isolated from 4 patients all produced quantities of IL-6 and IL-8. The concentrations of IL-6 in the medium after 7 days in culture ranged from 79,000 to 2,740,000 pg/ml, and the concentrations of IL-8 ranged from 40,000 to 1,000,000 pg/ml. Exposure of cyclosporin A and dexamethasone almost completely inhibited the production of IL-6 and IL-8 after 24 hours of treatment. Tacrolimus inhibited the production of both cytokines by 55%, while 15-deoxyspergualin reduced IL-6 levels by 24% and IL-8 levels by 48% after separate 24 hour treatments. These results suggest that these newer immunosuppressive agents may be useful in reducing the production of IL-6 and IL-8 in patients with cardiac myxoma.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1078-0297
|
| pubmed:author |
pubmed-author:HiraoYY,
pubmed-author:KandaTT,
pubmed-author:KobayashiII,
pubmed-author:LIT CTC,
pubmed-author:MasudaHH,
pubmed-author:NagaiRR,
pubmed-author:NegishiMM,
pubmed-author:OgishiKK,
pubmed-author:OhyamaYY,
pubmed-author:SakamakiTT,
pubmed-author:SakamotoHH,
pubmed-author:SawadaYY,
pubmed-author:SuminoHH
|
| pubmed:issnType |
Print
|
| pubmed:volume |
97
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
60-6
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9507569-Aged,
pubmed-meshheading:9507569-Female,
pubmed-meshheading:9507569-Heart Neoplasms,
pubmed-meshheading:9507569-Humans,
pubmed-meshheading:9507569-Immunohistochemistry,
pubmed-meshheading:9507569-Immunosuppressive Agents,
pubmed-meshheading:9507569-Interleukin-6,
pubmed-meshheading:9507569-Interleukin-8,
pubmed-meshheading:9507569-Kinetics,
pubmed-meshheading:9507569-Male,
pubmed-meshheading:9507569-Middle Aged,
pubmed-meshheading:9507569-Myxoma,
pubmed-meshheading:9507569-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Immunosuppressive drugs inhibit the production of interleukin-6 and interleukin-8 in cultured cardiac myxoma cells.
|
| pubmed:affiliation |
Second Department of Internal Medicine, Gunma University School of Medicine, Japan. sakamoth@news.sb.gunma-u.ac.jp
|
| pubmed:publicationType |
Journal Article
|