Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-3-27
pubmed:abstractText
Gingival crevicular fluid (GCF) was collected from 16 molar and premolar sites in each of 20 chronic periodontitis patients before and after periodontal therapy using filter paper strips. These were eluted individually into buffer for determination of cathepsin B and its endogenous inhibitors, alpha2-macroglobulin and cystatin. Cathepsin B activity was assayed with a fluorogenic peptide substrate, alpha2-macroglobulin by enzyme-linked immunosorbent assay and cystatin activity by inhibition of papain. Total amounts of enzyme and inhibitor per GCF sample decreased after treatment and correlated positively with pocket depth and gingival, bleeding and plaque indices. These comparisons were nearly always statistically significant for pooled site data and sometimes so for mean patient values. The amounts of alpha2-macroglobulin and cystatin were greater than those of cathepsin B and, surprisingly, enzyme and inhibitor levels correlated positively with each other. Experiments with purified reagents, however, demonstrated that the cathepsin B: alpha2-macroglobulin complex was still active against the low molecular weight substrate and that cystatin levels in GCF are probably insufficient to inhibit the enzyme substantially These factors may explain why GCF cathepsin B activity reflects the clinical status of periodontal lesions and has been identified in another study as a promising indicator of disease progression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0303-6979
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
34-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9477018-Adult, pubmed-meshheading:9477018-Biological Markers, pubmed-meshheading:9477018-Cathepsin B, pubmed-meshheading:9477018-Chronic Disease, pubmed-meshheading:9477018-Cystatins, pubmed-meshheading:9477018-Dental Plaque Index, pubmed-meshheading:9477018-Dental Scaling, pubmed-meshheading:9477018-Disease Progression, pubmed-meshheading:9477018-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:9477018-Female, pubmed-meshheading:9477018-Fluoroimmunoassay, pubmed-meshheading:9477018-Gingival Crevicular Fluid, pubmed-meshheading:9477018-Gingival Hemorrhage, pubmed-meshheading:9477018-Humans, pubmed-meshheading:9477018-Male, pubmed-meshheading:9477018-Middle Aged, pubmed-meshheading:9477018-Papain, pubmed-meshheading:9477018-Periodontal Pocket, pubmed-meshheading:9477018-Periodontitis, pubmed-meshheading:9477018-Root Planing, pubmed-meshheading:9477018-alpha-Macroglobulins
pubmed:year
1998
pubmed:articleTitle
Cathepsin B, alpha2-macroglobulin and cystatin levels in gingival crevicular fluid from chronic periodontitis patients.
pubmed:affiliation
Department of Stomatology, Third Clinical School of Beijing Medical University, China.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't