Linked Life Data

9473326

Source:http://linkedlifedata.com/resource/pubmed/id/9473326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-3-5
pubmed:abstractText
By voltage clamp technique and intracellular calcium measurements, we recorded in prawn oocytes simultaneous [Ca2+]i and ionic current changes stimulated by external Mg2+. The [Ca2+]i response consists of an oscillation period followed by a second state of sustained [Ca2+]i level. The oscillation period successively comprises a first [Ca2+]i peak, a series of [Ca2+]i transients, and a [Ca2+]i oscillatory plateau respectively concurrent with an initial transient outward K+Ca current, an inward Na+Ca current, and a final K+Ca outward current. By using inhibitor (heparin) or sensitizers (thimerosal or caffeine) of calcium release ER channels, and caged InsP3, we established that InsP3 is the sole second messenger releasing Ca2+ from intracellular stores. By sequential substitutions and reapplications of external Ca2+, and using econazole (50 microM), a Ca2+ influx inhibitor, we documented Ca2+ influx during the [Ca2+]i oscillatory plateau. The intracellular Ca2+ store was depleted with thapsigargin (75-350 nM) in Ca2+-free ASW. Reapplication of external Ca2+ evoked a rise in [Ca2+]i, indicating a store-dependent capacitative Ca2+ influx, correlated with a K+Ca outward current increase. No measurable Ca2+ release-activated Ca2+ current (Icrac) could be detected, but was indirectly demonstrated using the sensitivity of the K+Ca channels to [Ca2+]i. We propose that the involvement of external Ca2+, in the physiological [Ca2+]i response of prawn oocytes to external Mg2+, consists of a store-dependent capacitative Ca2+ influx.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0012-1606
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Depletion of intracellular Ca2+ stores, mediated by Mg2+-stimulated InsP3 liberation or thapsigargin, induces a capacitative Ca2+ influx in prawn oocytes.
pubmed:affiliation
Observatoire Océanographique et de Biologie marine de Roscoff, UPR CNRS 9042, France. hgoudeau@sbr.sb-roscoff.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't