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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-3-6
|
| pubmed:abstractText |
In order to study mitotic homologous recombination in somatic Drosophila melanogaster cells in vitro and to learn more on the question how recombination is influenced by mutagens, a genetic system was developed where spontaneous and drug-induced recombination could be monitored. Two recombination reporter substrates were stably introduced in multiple copies into the genome of established D. melanogaster Schneider line 2 cells: one plasmid (pSB310) contained the 5' and 3' deleted neomycin phosphoribosyltransferase alleles neoL and neoR as direct repeats; the other (pSB485) contained similar deletions (lacZL and lacZR) of the beta-galactosidase gene (lacZ). Restoration of a functional neo gene upon mitotic recombination between homologous sequences allowed direct selection for the event, whereas recombination in single cells harbouring the integrated lacZ-based reporter plasmid was detected by histochemical staining or flow cytometric analysis (FACS). The neo-based construct in the clonal transgenic cell line 44CD4 showed a spontaneous recombination frequency of 2.9 x 10(-4), whereas the 485AD1 cell line harbouring the lacZ-based construct exhibited a frequency of 2.8 x 10(-4). The alkylating agents EMS and MMS and the clastogen mitomycin C were able to induce recombination in the 485AD1 cell line in a dose-dependent manner. The results obtained from these studies suggest that the transgenic cell lines are potentially useful tools for identifying agents which stimulate direct repeat recombination in somatic Drosophila cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ethyl Methanesulfonate,
http://linkedlifedata.com/resource/pubmed/chemical/Gentamicins,
http://linkedlifedata.com/resource/pubmed/chemical/Methyl Methanesulfonate,
http://linkedlifedata.com/resource/pubmed/chemical/Mitomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Mutagens,
http://linkedlifedata.com/resource/pubmed/chemical/antibiotic G 418,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0027-5107
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
395
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9-27
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9465910-Animals,
pubmed-meshheading:9465910-Cells, Cultured,
pubmed-meshheading:9465910-Chromosomes,
pubmed-meshheading:9465910-Drosophila melanogaster,
pubmed-meshheading:9465910-Drug Resistance,
pubmed-meshheading:9465910-Escherichia coli,
pubmed-meshheading:9465910-Ethyl Methanesulfonate,
pubmed-meshheading:9465910-Flow Cytometry,
pubmed-meshheading:9465910-Genes, Reporter,
pubmed-meshheading:9465910-Gentamicins,
pubmed-meshheading:9465910-Methyl Methanesulfonate,
pubmed-meshheading:9465910-Mitomycin,
pubmed-meshheading:9465910-Mitosis,
pubmed-meshheading:9465910-Mutagenicity Tests,
pubmed-meshheading:9465910-Mutagens,
pubmed-meshheading:9465910-Polymerase Chain Reaction,
pubmed-meshheading:9465910-Recombination, Genetic,
pubmed-meshheading:9465910-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9465910-Transgenes,
pubmed-meshheading:9465910-beta-Galactosidase
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
A genetic system to detect mitotic recombination between repeated chromosomal sequences in Drosophila Schneider line 2 cells.
|
| pubmed:affiliation |
Department of Genetics, Swiss Federal Institute of Technology (ETH) Zürich, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|