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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-2-20
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pubmed:databankReference |
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pubmed:abstractText |
The structure of the two N-terminal domains of the gene 3 protein of filamentous phages (residues 1-217) has been solved by multiwavelength anomalous diffraction and refined at 1.46 A resolution. Each domain consists of either five or eight beta-strands and a single alpha-helix. Despite missing sequence homology, their cores superimposed with a root-mean-square deviation of 2 A. The domains are engaged in extensive interactions, resulting in a horseshoe shape with aliphatic amino acids and threonines lining the inside, delineating the likely binding site for the F-pilus. The glycine-rich linker connecting the domains is invisible in the otherwise highly ordered structure and may confer flexibility between the domains required during the infection process.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1072-8368
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
140-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9461080-Amino Acid Sequence,
pubmed-meshheading:9461080-Capsid Proteins,
pubmed-meshheading:9461080-Crystallography, X-Ray,
pubmed-meshheading:9461080-DNA-Binding Proteins,
pubmed-meshheading:9461080-Inovirus,
pubmed-meshheading:9461080-Models, Molecular,
pubmed-meshheading:9461080-Molecular Sequence Data,
pubmed-meshheading:9461080-Peptide Library,
pubmed-meshheading:9461080-Protein Conformation,
pubmed-meshheading:9461080-Selenomethionine,
pubmed-meshheading:9461080-Sequence Homology, Amino Acid,
pubmed-meshheading:9461080-Viral Fusion Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
The structural basis of phage display elucidated by the crystal structure of the N-terminal domains of g3p.
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pubmed:affiliation |
Macromolecular Structure Laboratory, ABL-Basic Research Program, NCI-FCRDC, Frederick, Maryland 21702, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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