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rdf:type |
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lifeskim:mentions |
umls-concept:C0034815,
umls-concept:C0040649,
umls-concept:C0085940,
umls-concept:C0086418,
umls-concept:C0208973,
umls-concept:C0596290,
umls-concept:C1517892,
umls-concept:C1531432,
umls-concept:C1704666,
umls-concept:C1706383,
umls-concept:C1879547
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pubmed:issue |
6 Pt 1
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pubmed:dateCreated |
1998-2-9
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pubmed:abstractText |
We examined whether histamine could regulate cell proliferation and expression of the early response gene c-fos in HEK-293 cells stably transfected with the human H2 receptor (HEK-H2). Histamine stimulated [3H]thymidine incorporation [50% effective concentration (EC50) = 3.6 x 10(-6) M] in HEK-H2 cells in a cimetidine-sensitive manner and increased c-fos mRNA in a time-dependent fashion, reaching maximal induction after 30 min. Histamine induced luciferase activity in HEK-H2 cells transiently transfected with a construct containing the luciferase reporter gene (Luc) coupled to the serum response element (SRE) of the c-fos gene promoter (EC50 = 1.5 x 10(-6) M) or a plasmid containing the SRE core fragment (bases -320 to -298). The protein kinase C (PKC) inhibitor staurosporine and long-term pretreatment of HEK cells with phorbol ester inhibited the effect of histamine on PKC activation, SRE-Luc activity, and [3H]thymidine incorporation. We have demonstrated that activation of the human H2 receptor can lead to induction of c-fos gene transcription and cell proliferation through a PKC-dependent mechanism.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cimetidine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Histamine H2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Staurosporine,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C2037-45
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9435511-Cell Division,
pubmed-meshheading:9435511-Cell Line,
pubmed-meshheading:9435511-Cimetidine,
pubmed-meshheading:9435511-Cloning, Molecular,
pubmed-meshheading:9435511-Cyclic AMP,
pubmed-meshheading:9435511-Enzyme Activation,
pubmed-meshheading:9435511-Epidermal Growth Factor,
pubmed-meshheading:9435511-Epinephrine,
pubmed-meshheading:9435511-Forskolin,
pubmed-meshheading:9435511-Genes, Reporter,
pubmed-meshheading:9435511-Genes, fos,
pubmed-meshheading:9435511-Histamine,
pubmed-meshheading:9435511-Humans,
pubmed-meshheading:9435511-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:9435511-Kinetics,
pubmed-meshheading:9435511-Luciferases,
pubmed-meshheading:9435511-Promoter Regions, Genetic,
pubmed-meshheading:9435511-Protein Kinase C,
pubmed-meshheading:9435511-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9435511-Receptors, Histamine H2,
pubmed-meshheading:9435511-Recombinant Fusion Proteins,
pubmed-meshheading:9435511-Signal Transduction,
pubmed-meshheading:9435511-Staurosporine,
pubmed-meshheading:9435511-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9435511-Transcription, Genetic,
pubmed-meshheading:9435511-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
Activation of the human histamine H2 receptor is linked to cell proliferation and c-fos gene transcription.
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pubmed:affiliation |
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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