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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-2-12
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pubmed:abstractText |
Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup 1 isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of L. pneumophila from three outbreaks. Hence, IRS-PCR assay appears to be a reproducible (intergel reproducibility, 100%) and discriminative (discriminatory index, > or = 0.996) tool for typing of Legionella. Compared to PFGE, however, IRS-PCR presented an advantage through ease of performance and with attributes of rapidity and sensitivity of target DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-1320629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-1607704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-1890182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-1967319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-3069867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-6394609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7494007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7536215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7665634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7673683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7705490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7883887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7883910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-7923052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8248630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8370750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8394380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8576337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8735131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8782669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9431941-8880485
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
161-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1998
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pubmed:articleTitle |
Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains.
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pubmed:affiliation |
Centre National de Référence des Legionella, Laboratoire de Bactériologie, UPRES EA1655, Faculté de Médecine R.T.H. Laënnec, Lyon, France. derba@cimac-res.univ-lyon1.fr
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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