Linked Life Data

9431813

Source:http://linkedlifedata.com/resource/pubmed/id/9431813

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1998-3-5
pubmed:abstractText
The Drosophila melanogaster gene teashirt (tsh) is essential for segment identity of the embryonic thorax and abdomen. A deletion 3' to the tsh transcription unit causes the loss of tsh early expression in the even-numbered parasegments, and the corresponding larval cuticular patterns are disrupted. tsh function in the odd-numbered parasegments in these mutants is normal by both criteria. The in vivo activities of genomic fragments from the deleted region were tested in transgenic embryos. A 2.0 kb enhancer from the 3' region acts mainly in the even-numbered parasegments and is dependent on fushi tarazu (ftz) activity, which encodes a homeodomain protein required for the development of even-numbered parasegments. Ftz protein binds in vitro to four distinct sequences in a 220 bp sub-fragment; these and neighboring sequences are conserved in the equivalent enhancer isolated from Drosophila virilis. Tsh protein produced under the control of the 220 bp enhancer partially rescues a null tsh mutation, with its strongest effect in the even-numbered parasegments. Mutation of the Ftz binding sites partially abrogates the capacity for rescue. These results suggest a composite mechanism for regulation of tsh, with different activators such as ftz contributing to the overall pattern of expression of this key regulator.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-72
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9431813-Animals, pubmed-meshheading:9431813-Base Sequence, pubmed-meshheading:9431813-Binding Sites, pubmed-meshheading:9431813-Drosophila, pubmed-meshheading:9431813-Drosophila Proteins, pubmed-meshheading:9431813-Embryo, Nonmammalian, pubmed-meshheading:9431813-Enhancer Elements, Genetic, pubmed-meshheading:9431813-Fushi Tarazu Transcription Factors, pubmed-meshheading:9431813-Gene Expression Regulation, Developmental, pubmed-meshheading:9431813-Homeodomain Proteins, pubmed-meshheading:9431813-Molecular Sequence Data, pubmed-meshheading:9431813-Mutation, pubmed-meshheading:9431813-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:9431813-Repressor Proteins, pubmed-meshheading:9431813-Sequence Homology, Nucleic Acid, pubmed-meshheading:9431813-Transcription, Genetic, pubmed-meshheading:9431813-Transcription Factors
pubmed:year
1997
pubmed:articleTitle
Transcriptional regulation of the Drosophila homeotic gene teashirt by the homeodomain protein Fushi tarazu.
pubmed:affiliation
Laboratoire de Génétique et Physiologie du Développement, UMR 9943 C.N.R.S.-Université, I.B.D.M. CNRS-INSERM-Université de la Méditerranée, Marseille, France.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't