Linked Life Data

9409684

Source:http://linkedlifedata.com/resource/pubmed/id/9409684

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1998-1-22
pubmed:databankReference
pubmed:abstractText
In vertebrates, transcriptional control of skeletal muscle genes during differentiation is regulated by enhancers that direct the combinatorial binding and/or interaction of MEF2 and the bHLH MyoD family of myogenic factors. We have shown that Drosophila MEF2 plays a role similar to its vertebrate counterpart in the regulation of the Tropomyosin I gene in the development of Drosophila somatic muscles, however, unlike vertebrates, Drosophila MEF2 interacts with a muscle activator region that does not have binding sites for myogenic bHLH-like factors or any other known Drosophila transcription factors. We describe here the isolation and characterization of a component of the muscle activator region that we have named PDP1 (PAR domain protein 1). PDP1 is a novel transcription factor that is highly homologous to the PAR subfamily of mammalian bZIP transcription factors HLF, DBP and VBP/TEF. This is the first member of the PAR subfamily of bZIP transcription factors to be identified in Drosophila. We show that PDP1 is involved in regulating expression of the Tropomyosin I gene in somatic body-wall and pharyngeal muscles by binding to DNA sequences within the muscle activator that are required for activator function. Mutations that eliminate PDP1 binding eliminate muscle activator function and severely reduce expression of a muscle activator plus MEF2 mini-enhancer. These and previous results suggest that PDP1 may function as part of a larger protein/DNA complex that interacts with MEF2 to regulate transcription of Drosophila muscle genes. Furthermore, in addition to being expressed in the mesoderm that gives rise to the somatic muscles, PDP1 is also expressed in the mesodermal fat body, the developing midgut endoderm, the hindgut and Malpighian tubules, and the epidermis and central nervous system, suggesting that PDP1 is also involved in the terminal differentiation of these tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4685-96
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9409684-Amino Acid Sequence, pubmed-meshheading:9409684-Animals, pubmed-meshheading:9409684-Animals, Genetically Modified, pubmed-meshheading:9409684-Base Sequence, pubmed-meshheading:9409684-Basic-Leucine Zipper Transcription Factors, pubmed-meshheading:9409684-Binding Sites, pubmed-meshheading:9409684-DNA, Complementary, pubmed-meshheading:9409684-DNA-Binding Proteins, pubmed-meshheading:9409684-Drosophila, pubmed-meshheading:9409684-Drosophila Proteins, pubmed-meshheading:9409684-Ectoderm, pubmed-meshheading:9409684-Endoderm, pubmed-meshheading:9409684-Enhancer Elements, Genetic, pubmed-meshheading:9409684-G-Box Binding Factors, pubmed-meshheading:9409684-Gene Expression Regulation, Developmental, pubmed-meshheading:9409684-Genes, Insect, pubmed-meshheading:9409684-Humans, pubmed-meshheading:9409684-Insect Proteins, pubmed-meshheading:9409684-Mesoderm, pubmed-meshheading:9409684-Molecular Sequence Data, pubmed-meshheading:9409684-Muscle, Skeletal, pubmed-meshheading:9409684-Muscle Development, pubmed-meshheading:9409684-Sequence Homology, Amino Acid, pubmed-meshheading:9409684-Transcription Factors, pubmed-meshheading:9409684-Transcriptional Activation, pubmed-meshheading:9409684-Tropomyosin
pubmed:year
1997
pubmed:articleTitle
PDP1, a novel Drosophila PAR domain bZIP transcription factor expressed in developing mesoderm, endoderm and ectoderm, is a transcriptional regulator of somatic muscle genes.
pubmed:affiliation
Department of Biochemistry and Molecular Biology M/C536, University of Illinois College of Medicine, Chicago 60612, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.