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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1998-1-9
|
| pubmed:abstractText |
Cumulus-oocyte complexes of domestic cats were classified by morphology of ooplasm (A = good, B = fair, C = poor) and cultured for 24 h in TCM 199 with gonadotrophins (eCG, FSH, hCG or FSH/hCG). More of type A oocytes (52%) underwent in vitro maturation (IVM) than of type B (41%) or type C (17%). The gonadotrophin source did not affect frequency of IVM of type A (50-53%) or type B (38-44%) oocytes, but IVM of type C oocytes in hCG or FSH/hCG (27%/19%) was about double that in eCG or FSH alone (13%/10%). After IVF, frequency of cleavage for type A (54%), B (41%) and C (26%) oocytes was similar to the IVM frequency of the equivalent type. After 7 days, development to the morula (M) stage in vitro was similar among types (47-58%); however, higher percentages of type A and B oocytes developed to blastocysts (Bl), 31% and 29%, respectively, than of type C (15%). After transfer of day 5 (n = 70) or 6 (n = 32) M and Bl to day 4 or 5 recipients in trial 1 (n = 4) and 2 (n = 3), respectively, the three recipients in trial 2 gave birth to four live kittens. Development in vitro to M of IVM/IVF embryos frozen in propanediol plus sucrose during early cleavage was similar (64-69%) to that of cohort controls (64%), but Bl formation was reduced (13-17% versus 32%). Damage to the zona pellucida after plunging into liquid nitrogen at -30 degrees C was higher (11%) than that of the embryos cooled at 10 degrees C min-1 from -30 degrees C to -150 degrees C before storage (2%).
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0449-3087
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
69-82
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| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9404273-Animals,
pubmed-meshheading:9404273-Cats,
pubmed-meshheading:9404273-Cell Survival,
pubmed-meshheading:9404273-Cells, Cultured,
pubmed-meshheading:9404273-Cryopreservation,
pubmed-meshheading:9404273-Culture Media,
pubmed-meshheading:9404273-Embryo, Mammalian,
pubmed-meshheading:9404273-Embryo Transfer,
pubmed-meshheading:9404273-Embryonic and Fetal Development,
pubmed-meshheading:9404273-Female,
pubmed-meshheading:9404273-Fertilization in Vitro,
pubmed-meshheading:9404273-Follicle Stimulating Hormone,
pubmed-meshheading:9404273-Gonadotropins, Equine,
pubmed-meshheading:9404273-Male,
pubmed-meshheading:9404273-Oocytes,
pubmed-meshheading:9404273-Oogenesis,
pubmed-meshheading:9404273-Pregnancy,
pubmed-meshheading:9404273-Reproductive Techniques
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
In vitro and in vivo development of embryos produced by in vitro maturation and in vitro fertilization of cat oocytes.
|
| pubmed:affiliation |
Center for Reproduction of Endangered Wildlife, Cincinnati Zoo, OH 45220, USA.
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| pubmed:publicationType |
Journal Article
|