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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3-4
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pubmed:dateCreated |
1998-1-5
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pubmed:abstractText |
Expression of microbial protein sequences in eukaryotic cells transfected by transcriptional/translational permissive cDNA constructs can induce systemic humoral and cellular responses in vivo. Two methods of in vivo transfection have been described to date. One method uses large quantities of naked DNA injected into skeletal muscle. The second method uses relatively small quantities of DNA complexed to gold particles for bollistic penetration of the plasma membrane of keratinocytes. The major disadvantage of the bolistic method is that instrumentation is required which is not generally available.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1380-2933
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
211-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9373349-Animals,
pubmed-meshheading:9373349-Bronchi,
pubmed-meshheading:9373349-DNA,
pubmed-meshheading:9373349-Epithelial Cells,
pubmed-meshheading:9373349-Female,
pubmed-meshheading:9373349-Gene Transfer Techniques,
pubmed-meshheading:9373349-Glycine,
pubmed-meshheading:9373349-HIV Antibodies,
pubmed-meshheading:9373349-HIV Envelope Protein gp120,
pubmed-meshheading:9373349-HIV Envelope Protein gp160,
pubmed-meshheading:9373349-HIV Infections,
pubmed-meshheading:9373349-Immunity, Mucosal,
pubmed-meshheading:9373349-Mice,
pubmed-meshheading:9373349-Mice, Inbred BALB C,
pubmed-meshheading:9373349-Models, Molecular,
pubmed-meshheading:9373349-Spermine,
pubmed-meshheading:9373349-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Induction of mucosal anti-HIV antibodies by facilitated transfection of airway epithelium with lipospermine/DNA complexes.
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pubmed:affiliation |
Department of Pathology, Vanderbilt University, Nashville, TN 37232, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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