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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-1-23
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001351,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001352,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001353,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001354,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001355,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001356,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001357,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001358,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001359,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001360,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001361,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001362,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001363,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001364,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001365,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001366,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001367,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001368,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001369,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001370,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001371,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001941,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001942,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001943,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001944,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001945,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AB001946
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pubmed:abstractText |
To examine the unique TCR repertoire in auto-immune-prone (NZB x NZW)F1 (B/WF1) mice, we analysed the Vbeta4 CDR3 region of TCRbeta chain in spleens of young (1 month old) and aged (6 month old) BALB/c and B/WF1 mice. Total RNA from spleens was used for cDNA synthesis and TCRVbeta4 PCR products were cloned and sequenced. Young B/WF1 mice showed high frequency (38.5%) of anionic amino acid residues at position beta100 in TCRVbeta4 chain compared to that (19.0%) in young BALB/c mice. Aged BALB/c mice and B/WF1 mice showed increase of frequency (38.1 and 51.9%, respectively) of anionic residues at beta100. These results indicate that Vbeta4-T cells that have anionic residues at beta100 in CDR3 region of TCRbeta chain increase with age in normal mice. Auto-immune prone mice show high frequency of anionic residues at beta100 in TCRVbeta4 chain even at the age of 1 month. These T cells may interact with cationic self-antigen(s) and might contribute to the onset and/or the progression of systemic autoimmunity in concert with other genetic elements in B/WF1 mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0165-2478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9373213-Amino Acid Sequence,
pubmed-meshheading:9373213-Animals,
pubmed-meshheading:9373213-Base Sequence,
pubmed-meshheading:9373213-Clone Cells,
pubmed-meshheading:9373213-DNA,
pubmed-meshheading:9373213-Female,
pubmed-meshheading:9373213-Male,
pubmed-meshheading:9373213-Mice,
pubmed-meshheading:9373213-Mice, Inbred BALB C,
pubmed-meshheading:9373213-Molecular Sequence Data,
pubmed-meshheading:9373213-Receptors, Antigen, T-Cell, alpha-beta
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pubmed:year |
1997
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pubmed:articleTitle |
Analysis of Vbeta4 T cell receptor CDR3 repertoire in BALB/c and (NZB x NZW)F1 mice.
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pubmed:affiliation |
Department of Immunology, Saga Medical School, Nabeshima, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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