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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-1-8
|
| pubmed:abstractText |
The cytoskeletal components of hamster oocytes, zygotes, and spontaneously activated parthogenotes were examined after immunocytochemical labeling. Microtubules were found only in the anastral, tangentially arranged second meiotic spindle of unfertilized oocytes. Taxol treatment of unfertilized oocytes greatly augmented astral microtubules in both the metaphase II spindle and the cortex. Disruption of the meiotic spindle microtubules with nocodazole resulted in cortical chromosomal scattering. During hamster sperm incorporation and pronuclear formation, no sperm aster was detected in association with the male DNA. Instead, a large overlapping array of microtubules assembled in the cortex. By mitosis, this interphase array disassembled and an anastral metaphase spindle formed. Microtubule and chromatin configurations were also imaged in hamster oocytes injected with human sperm. Astral microtubules were absent from the sperm centrosome. The implications of these results are discussed in relation to the hamster oocyte penetration assay, a test commonly used by in vitro fertilization clinics to demonstrate the fertilizing ability of human sperm. We conclude that since hamsters and humans follow different methods of centrosome inheritance, maternal and paternal, respectively, the hamster may be an inappropriate model for exploring microtubule and centrosomal defects in humans or for assaying postinsemination forms of human male fertility defects.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
967-75
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9369159-Animals,
pubmed-meshheading:9369159-Antineoplastic Agents,
pubmed-meshheading:9369159-Chromatin,
pubmed-meshheading:9369159-Cricetinae,
pubmed-meshheading:9369159-Diagnostic Imaging,
pubmed-meshheading:9369159-Female,
pubmed-meshheading:9369159-Fertilization,
pubmed-meshheading:9369159-Germ Cells,
pubmed-meshheading:9369159-Humans,
pubmed-meshheading:9369159-Insemination,
pubmed-meshheading:9369159-Male,
pubmed-meshheading:9369159-Mesocricetus,
pubmed-meshheading:9369159-Microscopy, Confocal,
pubmed-meshheading:9369159-Microtubules,
pubmed-meshheading:9369159-Mitosis,
pubmed-meshheading:9369159-Nocodazole,
pubmed-meshheading:9369159-Oocytes,
pubmed-meshheading:9369159-Paclitaxel,
pubmed-meshheading:9369159-Parthenogenesis,
pubmed-meshheading:9369159-Spermatozoa,
pubmed-meshheading:9369159-Zygote
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Microtubule organization and chromatin configurations in hamster oocytes during fertilization and parthenogenetic activation, and after insemination with human sperm.
|
| pubmed:affiliation |
Department of Zoology, University of Wisconsin, Madison 53706, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|