Linked Life Data

9353309

Source:http://linkedlifedata.com/resource/pubmed/id/9353309

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
1997-12-12
pubmed:abstractText
Two different yeast genes were identified that when overexpressed suppressed the low iron growth defect of a mutation in the endoplasmic reticulum iron binding enzyme methyl sterol oxidase. These genes were determined to be novel and highly related. The deduced amino acid sequences indicated that both were membrane proteins having two identical histidine-rich motifs. The predicted proteins, while not ABC transporters, are homologous to a widely distributed family of transition metal transporters present in all kingdoms. Subcellular fractionation and fluorescence microscopy localized these gene products to mitochondria. Based on this result we term these genes Mitochondrial Fe Transporters (MFT). Cells with disruptions in both genes show a growth defect on low iron medium, suggesting that these genes have redundant function and can affect cytosolic iron levels. Measurement of mitochondrial iron in cells grown in iron-rich medium overexpressing MFT1 or MFT2 show a 2-5-fold increase in iron compared with mitochondria from control cells. These results suggest that the mitochondria may act as a reservoir for iron that can be mobilized and used for cytosolic purposes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28485-93
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9353309-Amino Acid Sequence, pubmed-meshheading:9353309-Biological Transport, pubmed-meshheading:9353309-Carrier Proteins, pubmed-meshheading:9353309-Cation Transport Proteins, pubmed-meshheading:9353309-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9353309-Genes, Fungal, pubmed-meshheading:9353309-Green Fluorescent Proteins, pubmed-meshheading:9353309-Iron, pubmed-meshheading:9353309-Luminescent Proteins, pubmed-meshheading:9353309-Mitochondria, pubmed-meshheading:9353309-Mitochondrial Proteins, pubmed-meshheading:9353309-Molecular Sequence Data, pubmed-meshheading:9353309-Open Reading Frames, pubmed-meshheading:9353309-Saccharomyces cerevisiae, pubmed-meshheading:9353309-Saccharomyces cerevisiae Proteins, pubmed-meshheading:9353309-Sequence Homology, Amino Acid
pubmed:year
1997
pubmed:articleTitle
Characterization of two homologous yeast genes that encode mitochondrial iron transporters.
pubmed:affiliation
Division of Cell Biology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.