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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1997-10-17
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pubmed:databankReference |
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pubmed:abstractText |
Locus control regions (LCRs) are thought to provide a dominant tissue-specific open chromatin domain that allows for proper gene regulation by enhancers/silencers and their associated transcription factors. Expression of the T-cell receptor alpha (TCR alpha) gene is limited to T cells and its locus exists in different chromatin configurations in expressing and nonexpressing cell types. Here we show that eight DNase I-hypersensitive sites in the TCR alpha locus comprise an LCR that confers T-cell compartment-specific expression upon a linked heterologous transgene. Removal of the three 5'-most hypersensitive sites of this LCR, containing TCR alpha enhancers/silencers, abolishes tissue-differential chromatin structure and results in transgene expression in all tissues examined. The remaining five DNase I-hypersensitive sites therefore constitute a novel control element possessing a widely active chromatin-opening function that allows for ubiquitous expression of a linked transgene in all transgenic founder mice. Furthermore, these data show that cis-acting elements without inherent LCR activity can dominantly modulate chromatin structure to determine tissue-specific gene expression in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-1731219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-1738600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2188673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2209539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2225071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2524381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2564317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2582491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2704733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2770542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2901953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-2992937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-3001531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-3478680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-3545063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-3773893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-3982505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-6327064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7635138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7774816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7828582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7859745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7889409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7958890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-7958909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8004678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8062832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8068937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8178371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8236459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8348617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8413235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8458325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8476572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8516848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8524268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8566752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8599090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8599939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8665857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8791532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-8858153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9305645-9121464
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5037-45
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9305645-Spleen,
pubmed-meshheading:9305645-Thymus Gland,
pubmed-meshheading:9305645-Ribonucleases,
pubmed-meshheading:9305645-Globins,
pubmed-meshheading:9305645-Deoxyribonuclease I,
pubmed-meshheading:9305645-Base Sequence,
pubmed-meshheading:9305645-Chromatin,
pubmed-meshheading:9305645-RNA, Messenger,
pubmed-meshheading:9305645-Models, Genetic,
pubmed-meshheading:9305645-Molecular Sequence Data,
pubmed-meshheading:9305645-T-Lymphocytes,
pubmed-meshheading:9305645-Gene Expression Regulation,
pubmed-meshheading:9305645-Transcription Factors,
pubmed-meshheading:9305645-Transgenes,
pubmed-meshheading:9305645-Enhancer Elements, Genetic
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