Linked Life Data

9300695

Source:http://linkedlifedata.com/resource/pubmed/id/9300695

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-10-8
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A55461, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF016462, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF016463, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF016464, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF016465, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/B55461, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M30311, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M31138, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M85193, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U31510, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X52082, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X65050, http://linkedlifedata.com/resource/pubmed/xref/PDB/P17981, http://linkedlifedata.com/resource/pubmed/xref/PDB/P17982, http://linkedlifedata.com/resource/pubmed/xref/PDB/P20974, http://linkedlifedata.com/resource/pubmed/xref/PDB/P52961, http://linkedlifedata.com/resource/pubmed/xref/PDB/Q03515
pubmed:abstractText
Several proteins with NAD+:arginine ADP-ribosyltransferase (ART) activity are expressed in T cells and affect their function. Rat T cells that express the ART designated RT6 are determinants of the expression of autoimmune diabetes. In the mouse, a 35-kDa ecto-ART modulates the proliferation and functional activity of CTL. Here we report on mouse ARTs designated Rt6-1 and Rt6-2 in BALB/c and C57BL/6 mice. mRNAs for Rt6-1 and Rt6-2 were found in spleen, thymus, and intestinal tissue of both strains, but Rt6-1 mRNA in C57BL/6 mice was detected only at low levels. Rt6-1 and Rt6-2 cDNAs from both strains were cloned and sequenced. Predicted amino acid sequences of Rt6-2 were identical in both strains, but there was an in-frame stop codon in the sequence of Rt6-1 in C57BL/6 mice not present in BALB/c mice. Recombinant C57BL/6 Rt6-2 and BALB/c Rt6-1 proteins expressed in COS1 cells exhibited ART activity and were documented to be glycosylphosphatidylinositol-linked membrane proteins. COS-1 cells transfected with a C57BL/6 Rt6-1 cDNA construct expressed a truncated protein consistent in size with that predicted by the presence of the stop codon. This approximately 21-kDa protein appeared not to be glycosylphosphatidylinositol linked to the cell surface and lacked ART activity. C57BL/6 Rt6-1 therefore appears to be a naturally occurring ART knockout. The expression of Rt6-1 and Rt6-2 mRNAs in lymphoid tissues suggests that these ARTs may regulate immune system functions. Expression of Rt6-2 or another redundant ART may compensate for the lack of enzymatically active Rt6-1 in C57BL/6 mice.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2741-9
pubmed:dateRevised
2009-9-7
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Expression in BALB/c and C57BL/6 mice of Rt6-1 and Rt6-2 ADP-ribosyltransferases that differ in enzymatic activity: C57BL/6 Rt6-1 is a natural transferase knockout.
pubmed:affiliation
Department of Medicine, University of Massachusetts Medical Center, Worcester 01655, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't