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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0007600,
umls-concept:C0013485,
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0039597,
umls-concept:C0185117,
umls-concept:C0205851,
umls-concept:C1280500,
umls-concept:C1511938,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1997-9-16
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pubmed:abstractText |
The teratocarcinoma-derived growth factor-1 (TDGF-1) gene codes for a 188-aminoacid glycoprotein that shares structural homology with the epidermal growth factor (EGF) family of growth factors. TDGF-1 is highly expressed in the undifferentiated embryonal carcinoma stem cell line NTERA2 clone D1 (NT2/D1) and its expression is downregulated in response to differentiating agents such as retinoic acid (RA) and hexamethylen-bisacetamide (HMBA). To assess the role of TDGF-1 in the onset and/or progression of human germ cell tumors, we analysed TDGF-1 expression by Northern blot and immunostaining in a panel of 59 human germ cell tumors of different histological origins. We show that TDGF-1 expression is markedly elevated in a subset of human testicular germ cell tumors as compared to normal testes. TDGF-1 overexpression occurs in about 100% of tumors with non-seminomatous phenotype, such as embryonal carcinomas and malignant undifferentiated teratocarcinomas. To address the questions of how TDGF-1 (previously called CRIPTO) may affect the growth and/or the differentiation of embryonal carcinoma cells, we have characterized the effects of exogenous recombinant TDGF-1 protein on the proliferation rate and differentiation 'potential of NT2/D1. Exogenous TDGF-1 protein stimulated DNA synthesis and cell proliferation in both undifferentiated and differentiated NT2/D1 cells. However, TDGF-1 protein treatment was unable to block differentiation induced by both RA and HMBA. These results suggest that TDGF-1 growth factor may represent an autocrine growth factor that may be involved in the process of development of testicular neoplasms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TDGF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0950-9232
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pubmed:author |
pubmed-author:ArmenanteFF,
pubmed-author:BaldassarreGG,
pubmed-author:De RosaGG,
pubmed-author:PaolettiII,
pubmed-author:PepeSS,
pubmed-author:PersicoM GMG,
pubmed-author:RambaldiMM,
pubmed-author:RomanoAA,
pubmed-author:SalvatoreGG,
pubmed-author:SandomenicoCC,
pubmed-author:StaibanoSS,
pubmed-author:VigliettoGG
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
927-36
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9285688-Amino Acid Sequence,
pubmed-meshheading:9285688-Blotting, Northern,
pubmed-meshheading:9285688-Carcinoma, Embryonal,
pubmed-meshheading:9285688-Cell Differentiation,
pubmed-meshheading:9285688-Cell Division,
pubmed-meshheading:9285688-Epidermal Growth Factor,
pubmed-meshheading:9285688-GPI-Linked Proteins,
pubmed-meshheading:9285688-Gene Expression,
pubmed-meshheading:9285688-Germinoma,
pubmed-meshheading:9285688-Growth Substances,
pubmed-meshheading:9285688-Humans,
pubmed-meshheading:9285688-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9285688-Male,
pubmed-meshheading:9285688-Membrane Glycoproteins,
pubmed-meshheading:9285688-Molecular Sequence Data,
pubmed-meshheading:9285688-Neoplasm Proteins,
pubmed-meshheading:9285688-Recombinant Proteins,
pubmed-meshheading:9285688-Teratocarcinoma,
pubmed-meshheading:9285688-Testicular Neoplasms,
pubmed-meshheading:9285688-Tumor Cells, Cultured,
pubmed-meshheading:9285688-Tumor Markers, Biological
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pubmed:year |
1997
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pubmed:articleTitle |
Expression of teratocarcinoma-derived growth factor-1 (TDGF-1) in testis germ cell tumors and its effects on growth and differentiation of embryonal carcinoma cell line NTERA2/D1.
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pubmed:affiliation |
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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