| pubmed-article:9278859 | pubmed:abstractText | We have cloned a full length cDNA coding for activin beta B subunit from the goldfish ovary. Sequence analysis of the goldfish activin beta B shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta B has 93 and 98% amino acid identity with that of human and zebrafish beta B subunit respectively. The identity of the cloned goldfish activin beta B was further confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta B is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish. | lld:pubmed |
