rdf:type |
|
lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0018038,
umls-concept:C0018067,
umls-concept:C0035696,
umls-concept:C0040300,
umls-concept:C0050668,
umls-concept:C0185117,
umls-concept:C0330390,
umls-concept:C1711351,
umls-concept:C2911684
|
pubmed:issue |
1
|
pubmed:dateCreated |
1997-10-10
|
pubmed:databankReference |
|
pubmed:abstractText |
We have cloned a full length cDNA coding for activin beta B subunit from the goldfish ovary. Sequence analysis of the goldfish activin beta B shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta B has 93 and 98% amino acid identity with that of human and zebrafish beta B subunit respectively. The identity of the cloned goldfish activin beta B was further confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta B is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0952-5041
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
19
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
37-45
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:9278859-Activins,
pubmed-meshheading:9278859-Amino Acid Sequence,
pubmed-meshheading:9278859-Animals,
pubmed-meshheading:9278859-Base Sequence,
pubmed-meshheading:9278859-CHO Cells,
pubmed-meshheading:9278859-Cloning, Molecular,
pubmed-meshheading:9278859-Cricetinae,
pubmed-meshheading:9278859-DNA, Complementary,
pubmed-meshheading:9278859-Goldfish,
pubmed-meshheading:9278859-Humans,
pubmed-meshheading:9278859-Molecular Sequence Data,
pubmed-meshheading:9278859-Mutagenesis, Site-Directed,
pubmed-meshheading:9278859-Oligopeptides,
pubmed-meshheading:9278859-Peptides,
pubmed-meshheading:9278859-RNA, Messenger,
pubmed-meshheading:9278859-Sequence Homology, Amino Acid
|
pubmed:year |
1997
|
pubmed:articleTitle |
Cloning of cDNA for goldfish activin beta B subunit, and the expression of its mRNA in gonadal and non-gonadal tissues.
|
pubmed:affiliation |
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|