Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-10-10
pubmed:databankReference
pubmed:abstractText
We have cloned a full length cDNA coding for activin beta B subunit from the goldfish ovary. Sequence analysis of the goldfish activin beta B shows that this peptide is extremely conserved across vertebrates. The mature region of goldfish activin beta B has 93 and 98% amino acid identity with that of human and zebrafish beta B subunit respectively. The identity of the cloned goldfish activin beta B was further confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activity of activin in the culture medium using F5-5 cell assay. mRNA of goldfish activin beta B is expressed in a variety of goldfish tissues including ovary, testis, brain, pituitary, kidney and liver, suggesting a wide range of physiological roles for activin in the goldfish.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0952-5041
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Cloning of cDNA for goldfish activin beta B subunit, and the expression of its mRNA in gonadal and non-gonadal tissues.
pubmed:affiliation
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't