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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1997-10-15
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pubmed:abstractText |
A plasmid pLTR-DT which contained a gene for diphtheria toxin A-chain (DT-A) under the control of the long terminal repeat (LTR) of bovine leukemia virus (BLV) (BLV-LTR) in the multicloning site of pUC-18 was entrapped in cationic liposomes composed of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dioleoyl phosphatidylethanolamine (DOPE) and dilauroyl phosphatidylcholine (DLPC) (1:2:2, molar ratio) (TMAG-liposome), and their antitumor effect on BLV-infected tumor cells was examined in vivo. The cationic TMAG-liposome containing pLTR-DT was successively injected into the tumor transplanted to nude mice. The growth of tumor was significantly inhibited by the injection of cationic TMAG-liposome containing pLTR-DT. On the other hand, TMAG-liposome containing pUC18 plasmids showed no such effect. These results suggest that a DT-A expression plasmid under the control of BLV-LTR is highly toxic to the BLV-infected tumor cells, and that the cationic liposomes, such as TMAG-liposome, may be efficient transfection reagent for BLV-infected tumor cells and can be utilized for DT-A gene delivery into BLV-infected tumor cells in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-dielaidoylphosphatidylethanolami...,
http://linkedlifedata.com/resource/pubmed/chemical/1,2-dilauroylphosphatidylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Diphtheria Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamates,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/didodecyl...,
http://linkedlifedata.com/resource/pubmed/chemical/diphtheria toxin fragment A
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0916-7250
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
617-9
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pubmed:dateRevised |
2006-5-1
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pubmed:meshHeading |
pubmed-meshheading:9271462-Animals,
pubmed-meshheading:9271462-Antineoplastic Agents,
pubmed-meshheading:9271462-Cattle,
pubmed-meshheading:9271462-Cells, Cultured,
pubmed-meshheading:9271462-Diphtheria Toxin,
pubmed-meshheading:9271462-Drug Carriers,
pubmed-meshheading:9271462-Enzootic Bovine Leukosis,
pubmed-meshheading:9271462-Gene Therapy,
pubmed-meshheading:9271462-Gene Transfer Techniques,
pubmed-meshheading:9271462-Glutamates,
pubmed-meshheading:9271462-Kidney,
pubmed-meshheading:9271462-Leukemia Virus, Bovine,
pubmed-meshheading:9271462-Liposomes,
pubmed-meshheading:9271462-Mice,
pubmed-meshheading:9271462-Mice, Nude,
pubmed-meshheading:9271462-Peptide Fragments,
pubmed-meshheading:9271462-Phosphatidylcholines,
pubmed-meshheading:9271462-Phosphatidylethanolamines,
pubmed-meshheading:9271462-Plasmids,
pubmed-meshheading:9271462-Recombinant Proteins,
pubmed-meshheading:9271462-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9271462-Sheep,
pubmed-meshheading:9271462-Transplantation, Heterologous
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pubmed:year |
1997
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pubmed:articleTitle |
In vivo antitumor effect of cationic liposomes containing diphtheria toxin A-chain gene on cells infected with bovine leukemia virus.
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pubmed:affiliation |
Department of Cell Chemistry, Institute of Cellular and Molecular Biology, Okayama University Medical School, Japan.
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pubmed:publicationType |
Journal Article
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