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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1997-9-2
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pubmed:abstractText |
The erythromegakaryocytic cell line (LAMA-84) and the erythroeosinophilic cell line (LAMA-87) were used to study receptor expression and receptor-mediated response to monocyte/macrophage colony-stimulating factor (M-CSF) and transforming growth factor beta (TGF-beta), two modulators of cell proliferation. As demonstrated by Northern blot analysis and reverse transcriptase polymerase chain reaction (RT-PCR), c-fms and M-CSF mRNA were expressed in both cell lines. M-CSF was detected in the supernatant of both cell lines and addition of a neutralizing anti-M-CSF antibody inhibited cell growth. The two LAMA cell lines were found to express TGF-beta1, -beta2, and -beta3 mRNAs and to secrete TGF-beta mostly in latent form. Addition of anti-TGF-beta antibodies to the culture medium increased their proliferation, whereas TGF-beta1 inhibited cell proliferation by downregulating the c-myc mRNA. These results show that the proliferation of both LAMA cell lines is positively and negatively regulated by autocrine mechanisms, implying the presence of M-CSF and TGF-beta, respectively. They suggest that similar autocrine loops could be involved in the growth regulation of leukemic cells in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0301-472X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
958-65
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9257809-Animals,
pubmed-meshheading:9257809-Blast Crisis,
pubmed-meshheading:9257809-Blotting, Northern,
pubmed-meshheading:9257809-Cell Division,
pubmed-meshheading:9257809-Culture Media, Conditioned,
pubmed-meshheading:9257809-Eosinophils,
pubmed-meshheading:9257809-Erythrocytes,
pubmed-meshheading:9257809-Humans,
pubmed-meshheading:9257809-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:9257809-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9257809-Megakaryocytes,
pubmed-meshheading:9257809-Mice,
pubmed-meshheading:9257809-Mice, Nude,
pubmed-meshheading:9257809-Neoplasm Transplantation,
pubmed-meshheading:9257809-Polymerase Chain Reaction,
pubmed-meshheading:9257809-RNA, Messenger,
pubmed-meshheading:9257809-RNA-Directed DNA Polymerase,
pubmed-meshheading:9257809-Transforming Growth Factor beta,
pubmed-meshheading:9257809-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Proliferation of LAMA-84 and LAMA-87 cell lines is modulated by autocrine loops involving M-CSF and TGF-beta.
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pubmed:affiliation |
Laboratoire de Cytologie, Centre Hospitalier Régional et Universitairede Grenoble, France.
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pubmed:publicationType |
Journal Article
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