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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010092,
umls-concept:C0010453,
umls-concept:C0017262,
umls-concept:C0018061,
umls-concept:C0018207,
umls-concept:C0025543,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0331858,
umls-concept:C0333117,
umls-concept:C0542341,
umls-concept:C1280500,
umls-concept:C1704640,
umls-concept:C1706515,
umls-concept:C1999216,
umls-concept:C2911684
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|
pubmed:issue |
1
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pubmed:dateCreated |
1997-9-11
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|
pubmed:abstractText |
Granulosa cells were prepared from follicular aspirates obtained at oocyte collection for in-vitro fertilization (IVF) and maintained in culture. Substantial loss of cells from the culture surface occurred in the absence of gonadotrophin when cells were maintained on a thin layer of extracellular matrix (ECM) using a defined, serum-free medium. This cell loss was clearly and significantly reduced in the presence of human chorionic gonadotrophin (HCG) by days 4-6 of culture, and occurred in conjunction with loss of ECM. Analysis of culture medium by zymography using gelatin as substrate demonstrated the presence of metalloproteinases (MMP), MMP-9 (gelatinase B) appearing as the predominant band. Measurement of overall gelatinase activity in culture media revealed a progressive fall in gelatinase expressed on a per cell basis in media from HCG-treated cultures and this was less marked in controls. This suppression of gelatinase activity was consistent with an observed increase in production of tissue inhibitor of metalloproteinase-1 (TIMP-1) by HCG-treated cells, which was significant by days 6-8 of culture. We speculate that stabilization of the ECM may be an important aspect of HCG action in the corpus luteum.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1360-9947
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26-30
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9238653-Cells, Cultured,
pubmed-meshheading:9238653-Chorionic Gonadotropin,
pubmed-meshheading:9238653-Collagenases,
pubmed-meshheading:9238653-Corpus Luteum Maintenance,
pubmed-meshheading:9238653-Culture Media,
pubmed-meshheading:9238653-DNA,
pubmed-meshheading:9238653-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9238653-Extracellular Matrix,
pubmed-meshheading:9238653-Female,
pubmed-meshheading:9238653-Gelatinases,
pubmed-meshheading:9238653-Glycoproteins,
pubmed-meshheading:9238653-Granulosa Cells,
pubmed-meshheading:9238653-Humans,
pubmed-meshheading:9238653-Matrix Metalloproteinase 9,
pubmed-meshheading:9238653-Metalloendopeptidases,
pubmed-meshheading:9238653-Pregnancy,
pubmed-meshheading:9238653-Statistics, Nonparametric,
pubmed-meshheading:9238653-Tissue Inhibitor of Metalloproteinases
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pubmed:year |
1996
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pubmed:articleTitle |
Effect of gonadotrophin on cell and matrix retention and expression of metalloproteinases and their inhibitor in cultured human granulosa cells modelling corpus luteum function.
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pubmed:affiliation |
University of Southampton, Princess Anne Hospital, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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