Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-7-31
pubmed:abstractText
Although circulating levels of soluble intercellular adhesion molecule-1 (sICAM-1) are frequently used as an indicator of the severity of different immune, inflammatory, or neoplastic diseases, little is known about the factors that govern plasma sICAM-1 concentration and its relationship to the membranous form of ICAM-1 (mICAM-1) expressed on vascular endothelial cells. Plasma sICAM-1 concentration (measured by enzyme-linked immunosorbent assay) and mICAM-1 expression (measured using the dual radiolabeled monoclonal antibody technique) in different vascular beds (eg, lung, small intestine, and spleen) were monitored in wild-type (C57BL) and ICAM-1-deficient mice, before and after administration of tumor necrosis factor (TNF)-alpha. In wild-type mice, TNF-alpha elicited time-dependent increases in lung and intestine mICAM-1 (plateau achieved at 12 hours), with a corresponding increase in plasma sICAM-1 (peaked at 5 hours and then declined). The initial increases in mICAM-1 and pulmonary leukocyte sequestration (measured as lung myeloperoxidase activity) induced by TNF-alpha preceded any detectable elevation in sICAM-1. In ICAM-1-deficient mice, plasma sICAM-1 was reduced by approximately 70%, with > 95% reductions of mICAM-1 in lung and intestine, and > 75% reduction in splenic accumulation of anti-ICAM-1 antibody. Although TNF-alpha doubled plasma sICAM-1 in ICAM-1-deficient mice, mICAM-1 was unaffected in all tissues. Either splenectomy or pretreatment with cycloheximide resulted in an attenuated TNF-induced increase in sICAM-1, without affecting mICAM-1 expression. These findings indicate that plasma sICAM-1 concentration does not accurately reflect the level of ICAM-1 expression on endothelial cells in different vascular beds.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1283598, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1355078, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1357985, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1676471, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1682385, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1696029, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1967552, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-1980124, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-2551036, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-2981916, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-3137261, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-637870, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7489978, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7506035, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7507411, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7511993, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7525560, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7525937, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7541009, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7561056, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7642752, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7643527, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7751650, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7848308, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-7962529, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8104338, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8199653, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8476577, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8594904, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8608897, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8617433, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8640995, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8641365, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8701848, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-8781489, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-9029122, http://linkedlifedata.com/resource/pubmed/commentcorrection/9212746-9040457
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
151
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
205-14
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Differential up-regulation of circulating soluble and endothelial cell intercellular adhesion molecule-1 in mice.
pubmed:affiliation
Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.