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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1997-7-15
|
| pubmed:abstractText |
Based on the observation that chromosome 1q deletions are not infrequent in late-stage human breast carcinomas, we tested whether the recently discovered human melanoma metastasis suppressor gene, KiSS-1, which maps to chromosome 1q32-q41, could suppress metastasis of the human breast carcinoma cell line MDA-MB-435. Parental, vector-only transfectants and KiSS-1 transfectant clones were injected into the mammary fat pads of athymic nude mice and assessed for tumor growth and spontaneous metastasis to regional lymph nodes and lungs. Expression of KiSS-1 reduced metastatic potential by 95% compared to control cells but did not suppress tumorigenicity. Metastasis suppression correlated with a decreased clonogenicity in soft (0.3%) and hard (0.9%) agar. Although the overall rate of cell adhesion to extracellular matrix components was unaffected, KiSS-1 transfectants spread on immobilized type-IV collagen more rapidly than did control populations. Invasion and motility were unaffected by KiSS-1. Based on the predicted structure of the KiSS-1 protein, our results imply a mechanism whereby KiSS-1 regulates events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. In addition to its already described role in melanoma, our results show that KiSS-1 also functions as a metastasis suppressor gene in at least some human breast cancers.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2384-7
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9192814-Animals,
pubmed-meshheading:9192814-Blotting, Northern,
pubmed-meshheading:9192814-Breast Neoplasms,
pubmed-meshheading:9192814-Cell Adhesion,
pubmed-meshheading:9192814-Cell Movement,
pubmed-meshheading:9192814-Female,
pubmed-meshheading:9192814-Genes, Tumor Suppressor,
pubmed-meshheading:9192814-Humans,
pubmed-meshheading:9192814-Kisspeptins,
pubmed-meshheading:9192814-Mice,
pubmed-meshheading:9192814-Mice, Nude,
pubmed-meshheading:9192814-Neoplasm Metastasis,
pubmed-meshheading:9192814-Neoplasm Transplantation,
pubmed-meshheading:9192814-Proteins,
pubmed-meshheading:9192814-RNA, Messenger,
pubmed-meshheading:9192814-Transfection,
pubmed-meshheading:9192814-Tumor Cells, Cultured,
pubmed-meshheading:9192814-Tumor Suppressor Proteins
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Suppression of metastasis in human breast carcinoma MDA-MB-435 cells after transfection with the metastasis suppressor gene, KiSS-1.
|
| pubmed:affiliation |
The Jake Gittlen Cancer Research Institute, The Pennsylvania State University College of Medicine, Hershey 17033, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|