Linked Life Data

9180158

Source:http://linkedlifedata.com/resource/pubmed/id/9180158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-6-26
pubmed:abstractText
The expression of urokinase-type plasminogen activator (u-PA), its receptor (u-PAR) and metalloproteases activity were analyzed in 4 human gastric-cancer cell lines (AGS, Hs746T, SNU-1, and SNU-5), in an attempt to relate these activities to their invasive potential and tumorigenicity on the modified chorioallantoic membranes (CAM) of chick embryos. Only 1 of the 4 cell lines tested, Hs746T, expressed both u-PA and u-PAR as well as MMP-2, but not MMP-9. This cell line was both tumorigenic and highly invasive (51.3 +/- 13.1%) on a modified CAM. Its invasive capacity was comparable with that of a highly malignant human epidermoid-carcinoma cell line (HEp3), which usually showed 40 to 50% invasiveness. The 3 other cell lines all produced MMP-2 and MMP-9, but only AGS showed moderate invasiveness (24.2 +/- 8.8%). While antibodies to u-PA were significantly effective in reducing CAM invasiveness of Hs746T cells by approximately 40%, the invasiveness of the t-PA-expressing AGS cell line was not affected by anti-t-PA antibodies. These results suggest that when one of the components of the u-PA/u-PAR system (the enzyme and/or the receptor) is not produced and u-PA/u-PAR-dependent cell-surface proteolytic activity is thereby diminished, the malignant phenotype that can be determined by tumorigenicity and invasion of connective tissue on a CAM is compromised. Production of both type-IV collagenases (MMP-2 and MMP-9) cannot offset this deficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0020-7136
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
867-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9180158-Animals, pubmed-meshheading:9180158-Blotting, Northern, pubmed-meshheading:9180158-Chick Embryo, pubmed-meshheading:9180158-Collagenases, pubmed-meshheading:9180158-Gelatinases, pubmed-meshheading:9180158-Gene Expression, pubmed-meshheading:9180158-Humans, pubmed-meshheading:9180158-Matrix Metalloproteinase 2, pubmed-meshheading:9180158-Matrix Metalloproteinase 9, pubmed-meshheading:9180158-Metalloendopeptidases, pubmed-meshheading:9180158-Neoplasm Invasiveness, pubmed-meshheading:9180158-Receptors, Cell Surface, pubmed-meshheading:9180158-Receptors, Urokinase Plasminogen Activator, pubmed-meshheading:9180158-Stomach Neoplasms, pubmed-meshheading:9180158-Tissue Plasminogen Activator, pubmed-meshheading:9180158-Tumor Cells, Cultured, pubmed-meshheading:9180158-Urokinase-Type Plasminogen Activator
pubmed:year
1997
pubmed:articleTitle
Co-expression of urokinase-type plasminogen activator and its receptor in human gastric-cancer cell lines correlates with their invasiveness and tumorigenicity.
pubmed:affiliation
Department of Microbiology and Cancer Research Center, Seoul National University College of Medicine, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't