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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1997-6-25
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pubmed:abstractText |
Recently c-Cbl has been reported to be phosphorylated upon CSF-1 stimulation. The product of the c-cbl proto-oncogene (c-Cbl) is a 120 kDa protein harboring several docking sites for Src homology 2 (SH2) domain containing proteins and proline-rich regions that have been shown to allow its constitutive association with the SH3 domains of Grb2. We demonstrate here that CSF-1 exposure of stable transfectant CHO cells expressing the CSF-1 receptor induced the sustained tyrosine phosphorylation of c-Cbl and its subsequent association with Crk-II and the p85 kDa subunit of the PI 3-kinase, while it constitutively associates with Grb2. We demonstrate by in vitro experiments that these associations require the SH2 domain of Crk-II and both the C- and N-terminal SH2 domains of the p85 subunit of the PI 3-kinase. cCbl is the major PI 3-kinase-containing protein in c-Fms expressing CHO cells upon CSF-1 stimulation. Thus c-Cbl behaves as a core protein, allowing the formation of a quaternary complex including, Crk-II, PI 3-kinase and Grb2. We provide evidence that this multiprotein complex can interact with the tyrosine phosphorylated CSF-1 receptor through the unoccupied SH2 domain of Grb2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/CBL protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GRB2 Adaptor Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GRB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-cbl,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-crk,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Colony-Stimulating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2331-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9178909-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:9178909-Animals,
pubmed-meshheading:9178909-CHO Cells,
pubmed-meshheading:9178909-Cricetinae,
pubmed-meshheading:9178909-GRB2 Adaptor Protein,
pubmed-meshheading:9178909-Humans,
pubmed-meshheading:9178909-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9178909-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:9178909-Phosphorylation,
pubmed-meshheading:9178909-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:9178909-Protein Binding,
pubmed-meshheading:9178909-Proteins,
pubmed-meshheading:9178909-Proto-Oncogene Proteins,
pubmed-meshheading:9178909-Proto-Oncogene Proteins c-cbl,
pubmed-meshheading:9178909-Proto-Oncogene Proteins c-crk,
pubmed-meshheading:9178909-Receptors, Colony-Stimulating Factor,
pubmed-meshheading:9178909-Tyrosine,
pubmed-meshheading:9178909-Ubiquitin-Protein Ligases,
pubmed-meshheading:9178909-src Homology Domains
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pubmed:year |
1997
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pubmed:articleTitle |
CSF-1 stimulation induces the formation of a multiprotein complex including CSF-1 receptor, c-Cbl, PI 3-kinase, Crk-II and Grb2.
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pubmed:affiliation |
INSERM U364, Faculte de Medecine, Nice, France.
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pubmed:publicationType |
Journal Article
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