Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-7-9
pubmed:abstractText
A general approach for isolating large nested deletions in P1 artificial chromosomes (PACs) and bacterial artificial chromosomes (BACs) by retrofitting with a loxP site-containing Tn10 mini-transposon is described. Cre-mediated recombination between the loxP site existing in these clones and one introduced by transposition leads to deletions and inversions of the DNA between these sites. Large deletions are selectively recovered by transducing the retrofitted PAC or BAC clones with P1 phage. The requirement that both loxP sites in the cointegrate be packaged into a P1 head ensures that only large deletions are rescued. PCR analyses identified these deletions as products of legitimate recombination between loxP sites mediated by Cre protein. BACs produce deletions much more efficiently than PACs although the former cannot be induced to greater than unit copy in cells. Mammalian cell-responsive antibiotic resistance markers are introduced as part of the transposon into genomic clone deletions for subsequent functional analysis. Most importantly, the loxP site retrofitting and P1 transduction can be performed in the same bacterial host containing these clones directly isolated from PAC or BAC libraries. These procedures should facilitate physical and functional mapping of genes and regulatory elements in these large plasmids.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-1314208, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-1421762, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-1528894, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-1549564, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-1764967, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-2738927, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-2847006, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-3033825, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-6220808, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-6230671, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7596828, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7710781, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7710783, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7776370, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7919652, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-7987390, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8136839, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8146166, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8231891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8255777, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8462094, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8796348, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-8880146, http://linkedlifedata.com/resource/pubmed/commentcorrection/9153322-9018325
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2205-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Isolating large nested deletions in bacterial and P1 artificial chromosomes by in vivo P1 packaging of products of Cre-catalysed recombination between the endogenous and a transposed loxP site.
pubmed:affiliation
Department of Medicine, SUNY Health Science Center, 750 East Adams Street, Syracuse, NY 13210, USA. chatterp@vax.cs.hscsyr.edu
pubmed:publicationType
Journal Article