Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-9-3
pubmed:databankReference
pubmed:abstractText
Reverse genetic analysis in Drosophila has been greatly aided by a growing collection of lethal P transposable element insertions that provide molecular tags for the identification of essential genetic loci. However, because the screens performed to date primarily have generated autosomal P-element insertions, this collection has not been as useful for performing reverse genetic analysis of X-linked genes. We have designed a reverse genetic screen that takes advantage of the hemizygosity of the X chromosome in males together with a cosmid-based transgene that serves as an autosomally linked duplication of a small region of the X chromosome. The efficacy and efficiency of this method is demonstrated by the isolation of mutations in Drosophila homologues of two well-studied genes, the human Neurofibromatosis 2 tumor suppressor and the yeast CDC42 gene. The method we describe should be of general utility for the isolation of mutations in other X-linked genes, and should also provide an efficient method for the isolation of new allcles of existing X-linked or autosomal mutations in Drosophila.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-1346755, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-1425358, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-1680377, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-1968635, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2116353, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2164028, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2195038, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2493993, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2555253, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2556711, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2830671, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-2924351, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-3125435, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-3125519, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-3199441, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-3209069, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7479892, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7559772, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7707974, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7720592, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7743921, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7906398, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7924981, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-7958857, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8005415, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8137424, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8162854, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8343959, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8382177, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8404527, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8504935, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8608920, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8625837, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8628256, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8636236, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8646773, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8666669, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8752207, http://linkedlifedata.com/resource/pubmed/commentcorrection/9136014-8946916
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
146
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
245-52
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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