Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-4-30
pubmed:abstractText
The GATA motif is a well known positive cis -regulatory element in vertebrates. In this work we report experimental evidence for the direct participation of a GATA motif in the expression of the Drosophila antibacterial peptide gene Cecropin A1 . Previously we have shown that a kappaB-like site is necessary for Cecropin A1 gene expression. Here we present evidence that the Drosophila Rel protein which binds to the kappaB-like site requires an intact GATA site for maximal Dif-mediated transactivation of the Cecropin A1 gene. We show that a Drosophila blood cell line contains factors binding specifically to the GATA motif of the Cecropin A1 gene. The GATA binding activity is likely to include member(s) of the GATA family of transcriptional regulators. We show that the promoters of several inducible insect immune genes possess GATA sites 0-12 base pairs away from kappaB-like sites in functionally important promoter regions. Clusters of GATA and kappaB sites are also observed in the promoters of two important mammalian immune genes, namely IL6 and IL3. The consistent proximity of GATA and kappaB sites appears to be a common theme in the immune gene expression of insects and mammals.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1408741, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1445351, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1541299, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1559609, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1638017, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1714909, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1740152, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1899226, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-1970293, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2001705, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2007608, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2018779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2104802, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2137552, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2247051, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2249770, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2275826, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2300555, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2390977, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2413021, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-2452654, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-3043231, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7550322, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7559649, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7612236, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7621828, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7772280, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7849506, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7916677, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-7916679, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8062131, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8114908, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8187633, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8228815, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8242747, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8321212, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8337755, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8345514, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8467806, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8607809, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-8816802, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-9012522, http://linkedlifedata.com/resource/pubmed/commentcorrection/9092634-9060393
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1233-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Adjacent GATA and kappa B-like motifs regulate the expression of a Drosophila immune gene.
pubmed:affiliation
Department of Molecular Biology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't