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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 2
|
| pubmed:dateCreated |
1997-4-28
|
| pubmed:abstractText |
Leukocyte rolling is commonly restricted to venules and mediated by selectins expressed both on leukocytes (L-selectin) and the vascular endothelium (P- and E-selectin). We show here that 2- to 3-h tumor necrosis factor-alpha (TNF-alpha) stimulation of the mouse cremaster muscle induces rolling in arterioles (diameters 30-70 microm; wall shear rates 225-1,770 s(-1)). Weak P-selectin expression was detected on arteriolar endothelium of TNF-alpha-stimulated cremaster muscles. No rolling was observed in arterioles smaller than 30 microm (wall shear rates 1,500-3,250 s(-1)). The arteriolar rolling flux fraction in wild-type mice averaged approximately 5% and rolling was blocked by the P-selectin monoclonal antibody (MAb) RB40.34. Rolling in L- and E-selectin-deficient mice was similar to that in wild-type mice and was also blocked by the MAb RB40.34. Rolling was completely absent in arterioles of P-selectin-deficient mice. The average rolling velocity in arterioles of wild-type and L-selectin-deficient mice was approximately 50 microm/s but increased to approximately 110 microm/s in E-selectin-deficient mice and after injection of the blocking E-selectin MAb 9A9 in wild-type mice. We conclude that TNF-alpha treatment induces P-selectin-dependent rolling in arterioles that requires E-selectin for rolling at normal velocities.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/P-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
272
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H1391-400
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9087616-Animals,
pubmed-meshheading:9087616-Antibodies, Monoclonal,
pubmed-meshheading:9087616-Arterioles,
pubmed-meshheading:9087616-COS Cells,
pubmed-meshheading:9087616-Cell Adhesion,
pubmed-meshheading:9087616-E-Selectin,
pubmed-meshheading:9087616-Endothelium, Vascular,
pubmed-meshheading:9087616-HL-60 Cells,
pubmed-meshheading:9087616-Humans,
pubmed-meshheading:9087616-L-Selectin,
pubmed-meshheading:9087616-Leukocytes,
pubmed-meshheading:9087616-Male,
pubmed-meshheading:9087616-Mice,
pubmed-meshheading:9087616-Mice, Inbred C57BL,
pubmed-meshheading:9087616-Mice, Knockout,
pubmed-meshheading:9087616-Muscle, Skeletal,
pubmed-meshheading:9087616-P-Selectin,
pubmed-meshheading:9087616-Recombinant Proteins,
pubmed-meshheading:9087616-Transfection,
pubmed-meshheading:9087616-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
TNF-alpha induces selectin-mediated leukocyte rolling in mouse cremaster muscle arterioles.
|
| pubmed:affiliation |
Department of Biomedical Engineering, University of Virginia School of Medicine, Charlottesville 22908, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|