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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-5-15
|
| pubmed:abstractText |
Altered prostanoid metabolism participates in the pathogenesis of diabetic complications. The rate-limiting enzyme in the control of prostanoid metabolism is constitutive cyclo-oxygenase (COX-1). This study examined the possibility that altered prostanoid metabolism derives from altered COX-1 expression in those tissues from diabetic rats, with characteristic changes in prostanoid production and related haemodynamics. This account also describes a procedure for estimation of minute amounts of COX-1 mRNA by reverse transcription and competitive polymerase chain reaction (RT-cPCR) amplification. In streptozotocin-diabetic rats (STZ-D, 55 mg/kg body weight), compared with age-matched controls, the level of COX-1 mRNA (in attomoles/micrograms tRNA +/- 1SD) was significantly decreased in sciatic nerve (0.50 +/- 0.26 versus 0.89 +/- 0.32 in controls; P < 0.05) and thoracic aorta (3.99 +/- 1.67 versus 8.80 +/- 2.37 in controls; P < 0.05). There were no differences in COX-1 mRNA in diabetic and control rat kidney and retina, though there was a trend towards increased expression with diabetes in the latter. Evening primrose oil (EPO) treatment increased COX-1 mRNA in nerve and retina to levels in diabetic rats that were higher than those of non-diabetic controls (1.21 +/- 0.28 for nerve and 0.065 +/- 0.017 for retina, where control retinae gave 0.031 +/- 0.020-see above for nerve). Treatment of diabetic rats with an aldose reductase inhibitor was without effect on COX-1 mRNA levels in the tissues examined. This study demonstrates that the changes in COX-1 mRNA levels in diabetic rats are organ specific and suggests that altered prostanoid metabolism can, in part, be explained by altered COX-1 expression. Apart from providing arachidonate as substrate for COX, EPO stimulates COX-1 expression in some tissues.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldehyde Reductase,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Efamol,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Essential,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Linoleic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/Ptgs1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Linolenic Acid
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0952-3278
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
56
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
157-63
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:9051726-Aldehyde Reductase,
pubmed-meshheading:9051726-Animals,
pubmed-meshheading:9051726-Aorta, Thoracic,
pubmed-meshheading:9051726-Blood Glucose,
pubmed-meshheading:9051726-Body Weight,
pubmed-meshheading:9051726-Cyclooxygenase 1,
pubmed-meshheading:9051726-Cyclooxygenase 2,
pubmed-meshheading:9051726-DNA Primers,
pubmed-meshheading:9051726-Diabetes Mellitus, Experimental,
pubmed-meshheading:9051726-Fatty Acids, Essential,
pubmed-meshheading:9051726-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9051726-Isoenzymes,
pubmed-meshheading:9051726-Linoleic Acids,
pubmed-meshheading:9051726-Male,
pubmed-meshheading:9051726-Membrane Proteins,
pubmed-meshheading:9051726-Plant Oils,
pubmed-meshheading:9051726-Polymerase Chain Reaction,
pubmed-meshheading:9051726-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:9051726-RNA, Messenger,
pubmed-meshheading:9051726-Rats,
pubmed-meshheading:9051726-Rats, Wistar,
pubmed-meshheading:9051726-Sciatic Nerve,
pubmed-meshheading:9051726-gamma-Linolenic Acid
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of constitutive cyclo-oxygenase (COX-1) in rats with streptozotocin-induced diabetes; effects of treatment with evening primrose oil or an aldose reductase inhibitor on COX-1 mRNA levels.
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| pubmed:affiliation |
Department of Pharmacology, St. Bartholomew's, London, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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