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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-1-23
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pubmed:abstractText |
Rabbit secretin, which differs from all other mammalian secretins in having a Leu residue in position 6 (instead of Phe) and a basic residue (Arg) in position 16, had a lower affinity than porcine secretion on recombinant rat secretin receptors but had a greater affinity than porcine secretin on recombinant rat VIP1 and PACAP I receptors. Synthetic [L6] porcine secretin had a reduced potency on secretin and VIP1 receptors whereas [R16] porcine secretin had a similar binding profile as rabbit secretin. Thus, an arginine residue in position 16 reduced 3-fold the affinity of secretin for secretin receptors but increased 30-fold its affinity for the VIP1 and PACAP I receptors. The introduction of an arginine residue in position 16, instead of glutamine, in VIP and PACAP had a similar effect: [R16] VIP and [R16] PACAP had 3- to 10-fold higher affinities than VIP and PACAP for VIP1 and PACAP I receptors, and 3-fold lower affinities for the secretin receptors. The three [R16] peptides also had a reduced potency on the chimeric receptor consisting of the N-terminal part of the secretin receptor grafted on the VIP1 receptor, and an enhanced potency on the chimeric receptor consisting of the N-terminal part of VIP1 receptor grafted on the secretin receptor, indicating that position 16 of each ligand interacted with the N-terminal extracellular domain of the receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adcyap1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Gastrointestinal Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vasoactive Intestinal...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vasoactive Intestinal...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Secretin,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoactive Intestinal Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/secretin receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
1314
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
267-73
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8982281-Animals,
pubmed-meshheading:8982281-Arginine,
pubmed-meshheading:8982281-CHO Cells,
pubmed-meshheading:8982281-Cricetinae,
pubmed-meshheading:8982281-Ligands,
pubmed-meshheading:8982281-Neuropeptides,
pubmed-meshheading:8982281-Pituitary Adenylate Cyclase-Activating Polypeptide,
pubmed-meshheading:8982281-Rabbits,
pubmed-meshheading:8982281-Rats,
pubmed-meshheading:8982281-Receptors, G-Protein-Coupled,
pubmed-meshheading:8982281-Receptors, Gastrointestinal Hormone,
pubmed-meshheading:8982281-Receptors, Peptide,
pubmed-meshheading:8982281-Receptors, Pituitary Adenylate Cyclase-Activating...,
pubmed-meshheading:8982281-Receptors, Pituitary Hormone,
pubmed-meshheading:8982281-Receptors, Vasoactive Intestinal Peptide,
pubmed-meshheading:8982281-Receptors, Vasoactive Intestinal Polypeptide, Type I,
pubmed-meshheading:8982281-Recombinant Fusion Proteins,
pubmed-meshheading:8982281-Secretin,
pubmed-meshheading:8982281-Swine,
pubmed-meshheading:8982281-Vasoactive Intestinal Peptide
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pubmed:year |
1996
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pubmed:articleTitle |
Effect of introduction of an arginine16 in VIP, PACAP and secretin on ligand affinity for the receptors.
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pubmed:affiliation |
Department of Biochemistry and Nutrition, Faculty of Medicine, Université Libre de Bruxelles, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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