Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-1-21
|
| pubmed:abstractText |
We investigated the role of TNF-alpha in the host defence mechanism against infection with a virulent strain of Cryptococcus neoformans. Administration of exogenous recombinant human TNF-alpha significantly prolonged the survival time of mice infected by intratracheal instillation of the organism. Surprisingly, neutralizing MoAb to murine TNF-alpha did not shorten their survival time, a finding inconsistent with previous results. To investigate the cause of this inconsistency, we examined the production of TNF-alpha in the lungs of infected mice. During the course of cryptococcosis, there was little or no generation of TNF-alpha mRNA in the lung. This might be partly due to a direct inhibitory action of the fungal microorganism of TNF-alpha production by macrophages. In vitro production of TNF-alpha by murine interferon-gamma (IFN-gamma)- and lipopolysaccharide (LPS)-stimulated macrophages was strongly inhibited by co-culturing with the whole yeast cells. In contrast, administration of recombinant murine IL-12 markedly induced TNF-alpha production and the neutralizing anti-TNF-alpha MoAb strongly blocked IL-12-induced protection of mice against cryptococcal infection. These results indicate that endogenously synthesized TNF-alpha has the potential to contribute to the elimination of C. neoformans and partly mediates the protective effect of IL-12.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0009-9104
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
106
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
468-74
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8973614-Animals,
pubmed-meshheading:8973614-Antibodies, Fungal,
pubmed-meshheading:8973614-Binding, Competitive,
pubmed-meshheading:8973614-Cryptococcosis,
pubmed-meshheading:8973614-Female,
pubmed-meshheading:8973614-Humans,
pubmed-meshheading:8973614-Interferon-gamma,
pubmed-meshheading:8973614-Interleukin-12,
pubmed-meshheading:8973614-Lipopolysaccharides,
pubmed-meshheading:8973614-Lung,
pubmed-meshheading:8973614-Macrophages,
pubmed-meshheading:8973614-Mice,
pubmed-meshheading:8973614-Mice, Inbred BALB C,
pubmed-meshheading:8973614-Mice, Inbred DBA,
pubmed-meshheading:8973614-Prognosis,
pubmed-meshheading:8973614-RNA, Messenger,
pubmed-meshheading:8973614-Recombinant Proteins,
pubmed-meshheading:8973614-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Contribution of tumour necrosis factor-alpha (TNF-alpha) in host defence mechanism against Cryptococcus neoformans.
|
| pubmed:affiliation |
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
|
| pubmed:publicationType |
Journal Article
|