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rdf:type |
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lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0005768,
umls-concept:C0012854,
umls-concept:C0019704,
umls-concept:C0032520,
umls-concept:C0178913,
umls-concept:C0370003,
umls-concept:C0680536,
umls-concept:C1511790,
umls-concept:C1521827,
umls-concept:C1521871,
umls-concept:C1707455,
umls-concept:C2347026
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-19
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pubmed:abstractText |
This study compared the performance of several in-house nested PCR systems and the Amplicor human immunodeficiency virus type 1 (HIV-1) PCR kit in the detection of HIV-1 DNA in Tanzanian samples prepared by two different methods. All six of the in-house primer sets evaluated had a higher sensitivity for HIV DNA detection in samples prepared by the Amplicor PCR sample preparation method than in those prepared by the Ficoll-Isopaque (FIP) density gradient centrifugation method. A sensitivity of 100% was achieved by combining two in-house primer sets. The sensitivity of the standard Amplicor HIV-1 PCR kit was only 59%, whereas a modified Amplicor HIV-1 PCR test had a sensitivity of 98%. Our data show that Tanzanian samples prepared by the Amplicor preparation method are more suitable for HIV-1 PCR testing than samples prepared by the FIP method. The modified, but not the standard, Amplicor HIV-1 PCR kit provides an alternative to the nested in-house PCR technique for the diagnosis of HIV infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1498766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1500744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1552213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-15566837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-15566852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1560339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1572969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1672165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1786144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-1863410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-2380380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-2403656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-2674182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-2716852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-2900353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-3336784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-3405290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-7738151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-7788424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-7888237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-7983718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-8308102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968925-8363755
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pubmed:keyword |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
278-80
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pubmed:dateRevised |
2009-11-18
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pubmed:otherAbstract |
PIP: Blood samples were collected from 73 pregnant mothers attending an antenatal clinic and from 14 adult females recruited into ongoing studies of the incidence and natural history of HIV-1 infection in Dar es Salaam, Tanzania. Study subjects were asymptomatic for HIV infection, but 65 tested HIV-positive. The authors compared the performance of several in-house nested polymerase chain reaction (PCR) systems and the Amplicor HIV-1 PCR kit in detecting HIV-1 DNA in the seropositive samples prepared by two different methods. All six of the in-house primer sets evaluated were more sensitive for HIV DNA detection in samples prepared by the Amplicor PCR sample preparation method than in those prepared by the Ficoll-Isopaque (FIP) density gradient centrifugation method. A sensitivity of 100% was achieved by combining two in-house primer sets. The sensitivity of the standard Amplicor HIV-1 PCR kit was only 59%, while a modified Amplicor HIV-1 PCR test had a sensitivity of 98%. These data indicate that Tanzanian samples prepared by the Amplicor preparation method are more suitable for HIV-1 PCR testing than samples prepared by the FIP method.
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pubmed:meshHeading |
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pubmed:year |
1997
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pubmed:articleTitle |
Comparison of in-house and commercial sample preparation and PCR amplification systems for detection of human immunodeficiency virus type 1 DNA in blood samples from Tanzanian adults.
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pubmed:affiliation |
Department of Microbiology/Immunology, Muhimbili University College of Health Sciences, University of Dar es Salaam, Tanzania.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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