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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-3-19
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pubmed:abstractText |
Apoptosis and syncytium formation are two mechanisms by which human immunodeficiency virus type 1 (HIV-1) impairs uninfected CD4+ T-cell function and are mainly involved in the progression of the disease to AIDS. Previously, we showed that gp120-containing, protease-deficient HIV-1 (L-2) particles generated syncytia by particle-mediated fusion with uninfected cultured CD4+ T cells. Here, we present evidence that such L-2 particles can induce apoptosis in 40 to 50% of T cells which were enriched from HIV-1-negative healthy donor-derived peripheral blood mononuclear cells (PBMC-Ts). Activation of PBMC-Ts with phytohemagglutinin, concanavalin A, or ionomycin after incubation with L-2 particles resulted in the loss of proliferative capacity and gradual induction of apoptosis over 3 days. Wild-type strain LAI particles or recombinant gp120 were markedly less efficient (< or = 15%) at inducing such apoptosis. Western blot (immunoblot) analysis revealed that L-2 particles contained a larger amount of Env gp120 than LAI particles. Either preincubation of PBMC-Ts with a Fas antagonist or preincubation of L-2 particles with soluble CD4 blocked most of the apoptosis. This suggests that L-2-like particles can play a major role in HIV-1-induced apoptosis of uninfected bystander cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1346269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1402655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1682474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1683728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1694865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-1835572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-2001175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-2452899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-2499113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7494257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7528780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7529365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7539037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7539892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7585008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7615007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7666547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7716549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7721085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7902858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-7957894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8096068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8096089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8219198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8455722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8579749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8968878-8599114
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8968878-Apoptosis,
pubmed-meshheading:8968878-Cells, Cultured,
pubmed-meshheading:8968878-Flow Cytometry,
pubmed-meshheading:8968878-HIV Envelope Protein gp120,
pubmed-meshheading:8968878-HIV-1,
pubmed-meshheading:8968878-Humans,
pubmed-meshheading:8968878-Lymphocyte Activation,
pubmed-meshheading:8968878-Recombinant Proteins,
pubmed-meshheading:8968878-T-Lymphocytes,
pubmed-meshheading:8968878-Virion
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pubmed:year |
1997
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pubmed:articleTitle |
Protease-defective, gp120-containing human immunodeficiency virus type 1 particles induce apoptosis more efficiently than does wild-type virus or recombinant gp120 protein in healthy donor-derived peripheral blood T cells.
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pubmed:affiliation |
Section of Serology, Hokkaido University, Sapporo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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