Linked Life Data

8954081

Source:http://linkedlifedata.com/resource/pubmed/id/8954081

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-1-9
pubmed:abstractText
Perforin, a potent pore-forming protein, plays an important role in lymphocyte-mediated cytolysis causing necrosis or, when combined with the granzymes, apoptosis. The studies on perforin, although already extensive, have been hampered by the limited amount of material available from killer lymphocytes. Using a cell line that expresses high levels of human perforin, we describe a straightforward purification scheme that allows isolation of the lytic protein in approximately 8 hours. Perforin is enriched from the YT-INDY cell line by cavitation followed by differential centrifugation and ion metal affinity chromatography. In addition to demonstrating the lytic activity of human perforin toward various cell lines, we show that the conditions of the cytotoxicity assay influence both the kinetics and magnitude of perforin-mediated cytotoxicity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
229
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
44-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Human perforin: rapid enrichment by immobilized metal affinity chromatography (IMAC) for whole cell cytotoxicity assays.
pubmed:affiliation
Department of Medicine, Evanston Hospital, Northwestern University, Evanston, Illinois 60201, USA. granzyme@merle.acns.nwu.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't