Linked Life Data

8933418

Source:http://linkedlifedata.com/resource/pubmed/id/8933418

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-2-27
pubmed:abstractText
Sensitive and specific radioimmunoassays (RIAs) have been developed and validated for the determination of stavudine, a nucleoside analog possessing anti-human immunodeficiency virus (HIV) activity, in human plasma and urine. The hemisuccinate of stavudine was conjugated with histamine and radioiodinated to yield the radiotracer. Antisera were produced by injecting the immunogen, stavudine-hemisuccinate-bovine thyroglobulin, into rabbits. The antisera exhibited high specificity for stavudine as the structurally related analogs and other anti-HIV agents did not interfere in the assays. The methods could reliably quantitate stavudine in plasma from 2.5-100 ng ml-1 and in urine from 5.0-1000 ng ml-1 (after 2.5-fold dilution) with good accuracy and precision. The lower limits of quantitation were 2.5 ng ml-1 in human plasma and 5.0 ng ml-1 in urine (after 2.5-fold dilution). The RIA methods were applied to the analysis of stavudine in plasma and urine obtained from HIV-infected patients receiving the drug in clinical trials.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Specific radioimmunoassays for the measurement of stavudine in human plasma and urine.
pubmed:affiliation
Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543, USA.
pubmed:publicationType
Journal Article, Comparative Study