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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-2-20
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned and characterized the two remaining unknown genes of the non-oxidative part of the pentose-phosphate pathway of Saccharomyces cerevisiae encoding the enzymes D-ribulose-5-phosphate 3-epimerase (Rpe1p) and D-ribose-5-phosphate ketol-isomerase (Rki1p). Rpe1p has an unexpected high specific activity of 2148 mU x (mg protein)-1 in crude extracts. Deletion mutants of RPE1 show no enzyme activity and are unable to grow on D-xylulose. Unexpectedly, haploid rki1 deletion mutants are not viable. Functional expression of RKI1 was demonstrated following an increase of gene dosage in the haploid rki1 deletion mutant, which restored viability and specific D-ribose-5-phosphate ketol-isomerase activity. Both enzymes show high similarity to the deduced protein sequences of various open reading frames, expressed sequence tags or cDNAs from different organisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0172-8083
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
404-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8929392-Aldose-Ketose Isomerases,
pubmed-meshheading:8929392-Automatic Data Processing,
pubmed-meshheading:8929392-Carbohydrate Epimerases,
pubmed-meshheading:8929392-Cloning, Molecular,
pubmed-meshheading:8929392-DNA, Complementary,
pubmed-meshheading:8929392-Gene Deletion,
pubmed-meshheading:8929392-Gene Dosage,
pubmed-meshheading:8929392-Gene Expression Regulation, Fungal,
pubmed-meshheading:8929392-Haploidy,
pubmed-meshheading:8929392-Open Reading Frames,
pubmed-meshheading:8929392-Plasmids,
pubmed-meshheading:8929392-Racemases and Epimerases,
pubmed-meshheading:8929392-Saccharomyces cerevisiae,
pubmed-meshheading:8929392-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8929392-Xylulose
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pubmed:year |
1996
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pubmed:articleTitle |
Cloning and characterization of the first two genes of the non-oxidative part of the Saccharomyces cerevisiae pentose-phosphate pathway.
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pubmed:affiliation |
Institut für Mikrobiologie und Genetik, Technische Hochschule Darmstadt, Schnittspahnstrasse 10, D-64287 Darmstadt, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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