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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1997-2-19
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| pubmed:abstractText |
We have previously shown that in highly enriched rat gastric parietal cells the intestinal peptide hormones oxyntomodulin and glucagon-like peptide-2 (GLP-2) compete for receptor-binding with glucagon-like peptide-1 (GLP-1), a potent cAMP-dependent stimulus of H+ production in vitro. It is, however, unknown whether oxyntomodulin and GLP-2 elicit a biological response by interacting with the GLP-1 receptor. Therefore, we used enriched rat parietal cells to investigate the effects of both hormones on the production of cAMP and H+ ([14C]aminopyrine accumulation). Both parameters were stimulated by oxyntomodulin in a concentration-dependent manner. EC50 values were 6.2.10(-8) and 2.5.10(-7) M oxyntomodulin for stimulation of H+ and cAMP production, respectively. The maximally effective concentrations for stimulation of [14C]aminopyrine accumulation and cAMP production were 1.10(-6) and 1.10(-5) M oxyntomodulin, respectively. At these concentrations oxyntomodulin was nearly as effective as 10(-4) M histamine and equally effective as 10(-8) M GLP-1 (7-36)NH2. In the enriched parietal cell preparation there was no immunocytochemical evidence of contaminating D cells. Accordingly, the responses to oxyntomodulin and GLP-1 (7-36)NH2 were not augmented by incubating the cells in the presence of a polyclonal anti-somatostatin antibody. [14C]Aminopyrine accumulation in response to oxyntomodulin was inhibited by the GLP-1 (7-36)NH2 receptor antagonist, exendin (9-39)NH2, but not by the H2-receptor antagonist, ranitidine. Oxyntomodulin and carbachol acted additively to stimulate [14C]aminopyrine accumulation. GLP-2 (10(-7) to 10(-5)M) was without effect on basal H+ and cAMP production; however, at 10(-5) M GLP-2 markedly inhibited oxyntomodulin-stimulated [14C]aminopyrine accumulation. It is concluded that, by interacting with parietal cell receptors for GLP-1 (7-36)NH2, oxyntomodulin, but not GLP-2, directly stimulates H+ production by activating the adenylate cyclase.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aminopyrine,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon-Like Peptide 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon-Like Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine H2 Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Oxyntomodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Ranitidine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/exendin (9-39) amide,
http://linkedlifedata.com/resource/pubmed/chemical/glucagon-like peptide 1 (7-36)amide,
http://linkedlifedata.com/resource/pubmed/chemical/glucagon-like peptide receptor
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0012-2823
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
398-405
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:8913701-Aminopyrine,
pubmed-meshheading:8913701-Animals,
pubmed-meshheading:8913701-Carbachol,
pubmed-meshheading:8913701-Cyclic AMP,
pubmed-meshheading:8913701-Dose-Response Relationship, Drug,
pubmed-meshheading:8913701-Female,
pubmed-meshheading:8913701-Glucagon,
pubmed-meshheading:8913701-Glucagon-Like Peptide 1,
pubmed-meshheading:8913701-Glucagon-Like Peptides,
pubmed-meshheading:8913701-Histamine,
pubmed-meshheading:8913701-Histamine H2 Antagonists,
pubmed-meshheading:8913701-Hydrogen-Ion Concentration,
pubmed-meshheading:8913701-Immunohistochemistry,
pubmed-meshheading:8913701-Oxyntomodulin,
pubmed-meshheading:8913701-Parietal Cells, Gastric,
pubmed-meshheading:8913701-Peptide Fragments,
pubmed-meshheading:8913701-Ranitidine,
pubmed-meshheading:8913701-Rats,
pubmed-meshheading:8913701-Rats, Wistar,
pubmed-meshheading:8913701-Receptors, Glucagon,
pubmed-meshheading:8913701-Somatostatin
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| pubmed:articleTitle |
Oxyntomodulin: a cAMP-dependent stimulus of rat parietal cell function via the receptor for glucagon-like peptide-1 (7-36)NH2.
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| pubmed:affiliation |
Department of Internal Medicine II, Technical University of Munich, Germany.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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