Linked Life Data

8887293

Source:http://linkedlifedata.com/resource/pubmed/id/8887293

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-3-10
pubmed:abstractText
Proteinuria represents one of the most unfavorable prognostic factors in the progression of nephropathies. Several lines of evidence support a role for proteinuria per se in the development of interstitial fibrosis, albeit the molecular mechanisms are still unknown. We investigated the potential role of integrins expressed on tubular cells in regulating the synthesis and organization of interstitial matrix or as mediators of tubulointerstitial damage in conditions mimicking the nephrotic milieu. Under basal conditions, cultured tubular cells highly expressed alpha 3 beta 1 and, at focal contacts, alpha v beta 3. In contrast, alpha v beta 5 was weakly and diffusely distributed all over the plasma membrane. Cultures on a variety of matrix substrates (fibronectin, laminin, collagen types I and IV, vitronectin, von Willebrand factor, fibrinogen) did not induce any phenotypic change in integrin expression by tubular cells. Conversely, the addition of albumin resulted in a highly increased membrane expression of beta 5, which was organized in typical focal contacts and was related to the dose of albumin added. Immunofluorescence, flow cytometry, immunoprecipitation and RT-PCR experiments argue for a complex mechanism that includes increased post-transcriptionally regulated protein synthesis, accelerated conversion of precursors to mature forms, and increased surface delivery to discrete adhesive structures. Up-regulation of the beta 5 chain in tubular cells was confirmed in 9 out of 11 kidney biopsies from proteinuric glomerulonephritides including membranous and focal sclerosing glomerulonephritis, while it was not expressed in nonproteinuric kidneys including five biopsy specimens. This is the first report indicating that proteinuria up-regulates the surface expression and distribution of a specific integrin chain on tubular cells. These observations suggest the participation of integrins in a hitherto unexplored mechanism of tubulo-interstitial responses to glomerular injury.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0085-2538
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1310-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8887293-Albuminuria, pubmed-meshheading:8887293-Antigens, CD, pubmed-meshheading:8887293-Biopsy, pubmed-meshheading:8887293-Cells, Cultured, pubmed-meshheading:8887293-Cytoskeletal Proteins, pubmed-meshheading:8887293-Extracellular Matrix Proteins, pubmed-meshheading:8887293-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:8887293-Humans, pubmed-meshheading:8887293-Immunoglobulin G, pubmed-meshheading:8887293-In Situ Hybridization, pubmed-meshheading:8887293-Integrin alphaV, pubmed-meshheading:8887293-Integrin beta Chains, pubmed-meshheading:8887293-Integrins, pubmed-meshheading:8887293-Kidney Tubules, pubmed-meshheading:8887293-Lipopolysaccharides, pubmed-meshheading:8887293-Nephrotic Syndrome, pubmed-meshheading:8887293-Proteinuria, pubmed-meshheading:8887293-Time Factors
pubmed:year
1996
pubmed:articleTitle
Tubulointerstitial responses in the progression of glomerular diseases: albuminuria modulates alpha v beta 5 integrin.
pubmed:affiliation
Division of Nephrology and Dialysis, University of Torino, Regina Margherita Children's Hospital, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't