Linked Life Data

8884774

Source:http://linkedlifedata.com/resource/pubmed/id/8884774

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-1-30
pubmed:abstractText
Repetitive local application of a short train of stimuli to the rat substantia nigra and ventral tegmental area elicited a predominant depolarizing, slow, long-lasting synaptic response in the dopaminergic cells intracellularly recorded in vitro. This slow excitatory postsynaptic potential ranged between 13 and 27 mV at holding potentials of about-75 mV and lasted for 0.2-6 s. It was not greatly affected by the perfusion of 6-cyano-7-nitroquinoxaline-2,3-dione (10-20 microM), while it was potentiated in the presence of bicuculline methiodide (30 microM) or picrotoxin (50-100 microM) and 2-hydroxysaclofen (100-300 microM). In contrast, a substantial component of the slow excitatory postsynaptic potential was reversibly depressed, in a concentration-dependent manner, by the application of the N-methyl-D-aspartate receptor antagonists D,1-2-amino-5-phosphonovalerate (10-100 microM). Furthermore, the slow excitatory postsynaptic potential was reversibly increased by the superfusion of nominally magnesium-free solution. It was graded, increasing in amplitude with increased stimulus intensity, and was blocked by tetrodotoxin (0.5 microM). We suggest that a sustained activation of synaptic terminals containing excitatory amino acids mediates a slow excitatory postsynaptic potential in the dopaminergic cells of the midbrain. N-Methyl-D-aspartate receptors participate in the generation of this slow potential, while the alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionate/kainate receptors do not seem to contribute substantially to this potential. This N-methyl-D-aspartate-mediated synaptic event could be implicated in the release of dopamine as well as in the excitotoxic injury of the dopaminergic neurons.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
785-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8884774-2-Amino-5-phosphonovalerate, pubmed-meshheading:8884774-6-Cyano-7-nitroquinoxaline-2,3-dione, pubmed-meshheading:8884774-Animals, pubmed-meshheading:8884774-Bicuculline, pubmed-meshheading:8884774-Dopamine, pubmed-meshheading:8884774-Electric Stimulation, pubmed-meshheading:8884774-Evoked Potentials, pubmed-meshheading:8884774-Excitatory Amino Acid Antagonists, pubmed-meshheading:8884774-Ketamine, pubmed-meshheading:8884774-Membrane Potentials, pubmed-meshheading:8884774-Mesencephalon, pubmed-meshheading:8884774-Neurons, pubmed-meshheading:8884774-Picrotoxin, pubmed-meshheading:8884774-Rats, pubmed-meshheading:8884774-Rats, Wistar, pubmed-meshheading:8884774-Receptors, N-Methyl-D-Aspartate, pubmed-meshheading:8884774-Substantia Nigra, pubmed-meshheading:8884774-Synapses, pubmed-meshheading:8884774-Synaptic Transmission, pubmed-meshheading:8884774-Tegmentum Mesencephali, pubmed-meshheading:8884774-Tetrodotoxin, pubmed-meshheading:8884774-Time Factors
pubmed:year
1996
pubmed:articleTitle
N-methyl-D-aspartate receptors mediate a slow excitatory postsynaptic potential in the rat midbrain dopaminergic neurons.
pubmed:affiliation
Clinica Neurologica Dip. Sanita' Pubblica, Universita' di Roma Tor Vergata, Italy.
pubmed:publicationType
Journal Article, In Vitro